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The oxidative stress 3-like protein GsOS3L, substrate of GsSnRK1, enhances salt and cadmium stresses in soybean roots

文献类型： 外文期刊

作者： Sun, Xiaohuan 1 ; Bu, Haidong 1 ; Lu, Haoran 2 ; Zhang, Junfeng 4 ; Li, Qiang 2 ; Xiao, Jialei 2 ; Ding, Xiaodong 2 ; Cao, Lei 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang, Peoples R China

2.Northeast Agr Univ, Coll Life Sci, Key Lab Agr Biol Funct Genes, Harbin, Peoples R China

3.Xinjiang Agr Univ, Coll Agr, Urumqi, Peoples R China

4.Harbin Univ, Harbin, Peoples R China

关键词： Wild soybean; GsOS3L; GsSnRK1; Phosphorylation; Palmitoylation; Abiotic stress

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2025 年 355 卷

页码：

收录情况： SCI

摘要： Salt and heavy metal stresses have significant impacts on crop growth and agricultural development. Wild soybean (Glycine soja) exhibits greater resistance to abiotic stresses than its cultivated counterpart (Glycine max). In this study, the oxidative stress 3-like gene GsOS3L was identified from yeast two hybridization cDNA library constructed from wild soybean RNA. 150 mM NaCl and 10 % PEG induced their expression in roots, stems and leaves, respectively. pGsOS3L:GUS activity was enhanced in various tissues with increasing NaCl and CdCl2 concentrations. Y2H, BiFC, and LCI activity assays revealed that GsOS3L interacted physically with the GsSnRK1 kinase. The GsOS3L protein, which contains potential phosphorylation and palmitoylation sites, was localized to the nucleus under normal conditions but translocated from the nucleus to the cell membrane under cadmium stress. This translocation was prevented by the palmitoylation inhibitor 2-bromopalmitate (2-BP) and by double mutation of the predicted palmitoylation sites (C4S/C21S). 2-BP treatment attenuated GsOS3L transgenic composite soybeans' cadmium stress tolerance. GsOS3L was found to be phosphorylated by GsSnRK1, which reduced the salt and cadmium stress tolerance of transgenic Arabidopsis plants. The findings of this study provide promising insights into the physiological and molecular mechanisms of GsOS3L in soybean under salt and cadmium stresses.

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