Phosphorylation of Wild Soybean (Glycine soja) Splicing Factor GsSCL30a by GsSnRK1 Regulates Soybean Tolerance to Alkali Stress
文献类型： 外文期刊
作者： Li, Minglong 1 ; Liu, Xin 1 ; Zhao, Xin 1 ; Liu, Fujing 1 ; Xue, Man 1 ; Liu, Haishan 1 ; Sun, Xiaohuan 3 ; Liu, Changyuan 3 ; Liu, Zhangxiong 4 ; Ding, Xiaodong 1 ; Li, Qiang 1 ; Xiao, Jialei 1 ;
作者机构： 1.Northeast Agr Univ, Key Lab Agr Biol Funct Genes, Harbin, Peoples R China
2.Northeast Agr Univ, Key Lab Soybean Biol Chinese Educ Minist, Harbin, Peoples R China
3.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang, Peoples R China
4.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improve, Key Lab Soybean Biol Beijing MOA,Key Lab Crop Gene, Beijing, Peoples R China
关键词： alkaline stress; cultivated soybean; GsSCL30a; splicing factor; wild soybean
期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.3； 五年影响因子：7.7 ）
ISSN： 0140-7791
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Pre-mRNA splicing is a fundamental process in plant gene expression, and the resulting transcripts may play significant roles in plant development and responses to environmental changes. Wild soybean (Glycine soja), as a close relative of cultivated soybean (Glycine max), has excellent traits such as alkali tolerance and is an ideal material for mining stress-resistant genes. Previous phosphoproteomic analysis identified SR protein GsSCL30a as a potential phosphorylation substrate of GsSnRK1 kinase. Y2H and BiFC assays confirmed their interaction. In vitro phosphorylation and mass spectrometry showed GsSnRK1 phosphorylates six residues in GsSCL30a. RT-qPCR revealed GsSCL30a is ubiquitously expressed, with alkali stress regulating its transcript levels. Alternative splicing analysis showed GsSCL30a acts as a splicing factor, self-splicing its third intron with conserved GAAG motifs and GsSnRK1 phosphorylation enhances this activity. GsSCL30a interacts with U1-70K, suggesting involvement in U1-RNP complex at 5 ' splice sites. Soybean hairy root overexpression showed GsSCL30a-GsSnRK1 synergistically enhances alkali tolerance. This study revealed the molecular mechanism of GsSnRK1-GsSCL30a module in response to alkali stress and provides a new idea to breed the high-resistant crops.
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