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Identification of sex determination region and candidate genes in blotched snakehead (Channa maculata) through genome-wide association studies and transcriptome analysis

文献类型： 外文期刊

作者： Liu, Haiyang 1 ; Cui, Tongxin 1 ; Zhang, Jin 1 ; Zhang, Yang 1 ; Luo, Qing 1 ; Fei, Shuzhan 1 ; Chen, Kunci 1 ; Zhao, Jian 1 ; Ou, Mi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Guangzhou 510380, Peoples R China

2.Zhejiang Ocean Univ, Sch Fishery, Zhoushan 316022, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China

4.Hunan Univ Sci & Technol, Sch Life & Hlth Sci, Xiangtan 411201, Peoples R China

关键词： Blotched snakehead; GWAS; Sex-determining; Sex-specific markers; Sex-related genes

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 42 卷

页码：

收录情况： SCI

摘要： The blotched snakehead (Channa maculata) is an economically significant freshwater species in China, exhibiting pronounced sexual size dimorphism, with males displaying faster growth rates and larger body sizes. Monosex farming has the potential to significantly substantially enhance aquaculture efficiency for this species. In this study, we conducted whole-genome resequencing of 45 female and 50 male blotched snakehead individuals. Genome-wide association analysis (GWAS) was performed based on genome-wide SNPs and InDels, and combined with fixation index (Fst) analysis between male and female groups, which identified a candidate sexdetermining region located on Chr.02: 34,288,075-34,351,554 (63.479 kb). Linkage disequilibrium analysis revealed that the sex-determining region has formed a haplotype block with a state of linkage disequilibrium. Within this region, we identified six InDels larger than 15 bp and developed two sex-specific markers capable of accurately identifying the genetic sex of blotched snakehead. Additionally, we performed transcriptome analyses of male and female gonads at 120, 150, and 180 days post-fertilization (dpf). Combined with the GWAS results, we identified candidate sex-determining genes, including five male-biased genes (EVM0022796, ITGB1BP1, RSAD2, CMPK2, and KIDINS220) and two female-biased genes (Sox11 and DNAJC30). This study enhances our understanding of the genetic basis of sex determination in blotched snakehead, providing novel insights into its genetic mechanisms. Our findings establish a solid scientific foundation for applications in monosex breeding and marker-assisted selection breeding programs in this species.

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