Four novel Cit7GlcTs functional in flavonoid 7-O-glucoside biosynthesis are vital to flavonoid biosynthesis shunting in citrus
文献类型： 外文期刊
作者： Yuan, Ziyu 1 ; Li, Gu 1 ; Zhang, Huixian 1 ; Peng, Zhaoxin 1 ; Ding, Wenyu 1 ; Wen, Huan 1 ; Zhou, Hanxin 1 ; Zeng, Jiwu 3 ; Chen, Jiajing 1 ; Xu, Juan 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China
2.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.6； 五年影响因子：8.2 ）
ISSN： 2662-6810
年卷期： 2024 年 11 卷 6 期
页码：
收录情况： SCI
摘要： Citrus fruits have abundant flavonoid glycosides (FGs), an important class of natural functional and flavor components. However, there have been few reports about the modification of UDP-glycosyltransferases (UGTs) on flavonoids in citrus. Notably, in flavonoid biosynthesis, 7-O-glucosylation is the initial and essential step of glycosylation prior to the synthesis of flavanone disaccharides, the most abundant and iconic FGs in citrus fruits. Here, based on the accumulation of FGs observed at the very early fruit development stage of two pummelo varieties, we screened six novel flavonoid 7-O-glucosyltransferase genes (7GlcTs) via transcriptomic analysis and then characterized them in vitro. The results revealed that four Cg7GlcTs possess wide catalytic activities towards various flavonoid substrates, with CgUGT89AK1 exhibiting the highest catalytic efficiency. Transient overexpression of CgUGT90A31 and CgUGT89AK1 led to increases in FG synthesis in pummelo leaves. Interestingly, these two genes had conserved sequences and consistent functions across different germplasms. Moreover, CitUGT89AK1 was found to play a role in the response of citrus to Huanglongbing infection by promoting FG production. The findings improve our understanding of flavonoid 7-O-glucosylation by identifying the key genes, and may help improve the benefits of flavonoid biosynthesis for plants and humans in the future.
- 相关文献
作者其他论文 更多>>
-
A smartphone-based electrochemical sensing platform for the portable and simultaneous determination of flavonoids in Citri Reticulatae Pericarpium
作者：Xia, Hong-qi;Zhu, Congyi;Qiu, Diyang;Zeng, Jiwu
关键词：Portable electrochemical sensing platform; Laser induced graphene; Citri reticulatae pericarpium; Polymethoxylated flavones; Hesperidin
-
Flavor characterization of Citri Reticulatae Pericarpium (Citrus reticulata 'Chachiensis') with different aging years via sensory and metabolomic approaches
作者：Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Liu, Yuan;Wen, Huan;Kong, Jiatao;Hu, Zhehui;Zhang, Hongyan;Chen, Jiajing;Xu, Juan;Hu, Yang;Zeng, Jiwu;Chen, Xiangling
关键词：Guangchenpi (GCP); Aging process; Sensory evaluation; Flavor -related metabolites; Characteristic aroma compounds; Predictive modeling
-
Integrated untargeted metabolomic and transcriptomic analyses reveal OMT genes controlling polymethoxyflavonoid biosynthesis in the pericarp of Citrus reticulata 'Chachi'
作者：Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Mao, Genlin;Chen, Wanbing;Wu, Pingzhi;Zhang, Ruimin;Zhang, Man;Huang, Yongjing;Zeng, Jiwu;Li, Xinqi;Xu, Juan;Li, Xinqi;Xu, Juan
关键词：Citrus functional qualities; Polymethoxyflavonoid; Potential biomarkers; Multi-omics combined analysis; Gene expression
-
Bruceine D inhibits the growth of Spodoptera litura by inducing cell apoptosis in the midgut via an oxidative burst
作者：Zeng, Jiwu;Mao, Genlin;Xu, Yuhui;Zeng, Jiwu;Lu, Wei;Xu, Yuhui;Liu, Huan;Xu, Hanhong;Xu, Hanhong
关键词：Bruceine D; Spodoptera litura; growth and development; midgut; ROS; apoptosis
-
Combined analysis of GC-MS, RNA-seq and ATAC-seq elucidates the essential oils variation and terpenes biosynthesis in Citrus grandis 'Tomentosa'
作者：Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu;Fan, Ruiyi;Qiu, Diyang;Mao, Genlin;Zeng, Jiwu
关键词：Essential oils; GC-MS; Transcriptome; ATAC-seq; Terpenes variations; Transcriptional factors
-
The gap-free genome and multi-omics analysis of Citrus reticulata 'Chachi' reveal the dynamics of fruit flavonoid biosynthesis
作者：Zhu, Congyi;Wu, Pingzhi;Huang, Yongjing;Zhang, Ruimin;Fan, Zhengyan;Zeng, Jiwu;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng;You, Congjun;Yu, Chao;Gong, Jinli;Hu, Xiaoli;Sun, Xuepeng
关键词：
-
Antibacterial Activity of Ethanol Extract from Australian Finger Lime
作者：Zhang, Ruimin;Fan, Zhengyan;Zhu, Congyi;Huang, Yongjing;Wu, Pingzhi;Zeng, Jiwu
关键词：antibacterial effect; Australian finger lime (Citrus australasica L.); ethanol extract; minimum inhibitory concentration; phytochemicals
