Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR
文献类型: 外文期刊
作者: Ye, Xing 1 ; Zhang, Lili 1 ; Dong, Haiyan 2 ; Tian, Yuanyuan 1 ; Lao, Haihua 1 ; Bai, Junjie 1 ; Yu, Lingyun 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fish Breeding & Cultivat, Guangzhou 510380, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
3.Sun Ya
关键词: gene expression;Ctenopharyngodon idella.;Bacterial infection;Quantitative real-time PCR;Reference gene;Internet resource.
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
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收录情况: SCI
摘要: Expression of four reference genes of grass carp, including o-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1l), was studied in tissues of normal individuals and bacteria-infected individuals. EF1l had the most stable expressions followed by 18S rRNA then GAPDH; ACTB had the least stability. After being infected with bacteria, the grass carp showed minimal changes in expression levels of EF1l in the liver and head kidney, while ACTB had the most stable expressions in spleen but the least stable in liver. EF1l is thus the optimal reference gene in quantitative real-time PCR analysis to quantitate the expression levels of target genes in tissues of grass carp.
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