Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR
文献类型: 外文期刊
作者: Ye, Xing 1 ; Zhang, Lili 1 ; Dong, Haiyan 2 ; Tian, Yuanyuan 1 ; Lao, Haihua 1 ; Bai, Junjie 1 ; Yu, Lingyun 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fish Breeding & Cultivat, Guangzhou 510380, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
3.Sun Ya
关键词: gene expression;Ctenopharyngodon idella.;Bacterial infection;Quantitative real-time PCR;Reference gene;Internet resource.
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Expression of four reference genes of grass carp, including o-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA (18S) and elongation factor-1 alpha (EF1l), was studied in tissues of normal individuals and bacteria-infected individuals. EF1l had the most stable expressions followed by 18S rRNA then GAPDH; ACTB had the least stability. After being infected with bacteria, the grass carp showed minimal changes in expression levels of EF1l in the liver and head kidney, while ACTB had the most stable expressions in spleen but the least stable in liver. EF1l is thus the optimal reference gene in quantitative real-time PCR analysis to quantitate the expression levels of target genes in tissues of grass carp.
- 相关文献
作者其他论文 更多>>
-
Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod (Oxyeleotris lineolata)
作者:Fan, Jiajia;Ma, Dongmei;Zhu, Huaping;Lin, Minghui;Zhong, Zaixuan;Tian, Yuanyuan;Fan, Jiajia;Ma, Dongmei;Zhu, Huaping;Lin, Minghui;Zhong, Zaixuan;Tian, Yuanyuan
关键词:sleepy cod Oxyeleotris lineolata; sexual dimorphism; transcriptome; sex determination; gonadal differentiation
-
A TMT-Based Proteomic Analysis of Osmoregulation in the Gills of Oreochromis mossambicus Exposed to Three Osmotic Stresses
作者:Su, Huanhuan;Ma, Dongmei;Fan, Jiajia;Zhong, Zaixuan;Tian, Yuanyuan;Zhu, Huaping;Su, Huanhuan;Ma, Dongmei;Fan, Jiajia;Zhong, Zaixuan;Tian, Yuanyuan;Zhu, Huaping
关键词:TMT-based proteomics; gills; osmotic stresses;
Oreochromis mossambicus -
Effects of different feeding strategies for the frozen Antarctic krill meal on growth, muscle nutrition, digestive enzyme activity, immunity, molting, and gut microbiota structure of Cherax quadricarinatus
作者:Mai, Zhuang;Liu, Fubai;Yang, Tianyan;Mai, Zhuang;Wei, Jie;Wang, Yakun;Liu, Fubai;Li, Huarong;Su, Qiyao;Hong, Kunhao;Zhou, Qiaoyan;Jiao, Tianhui;Yu, Lingyun;Zeng, Zhongqiang;Li, Huarong;Su, Qiyao;Hong, Kunhao;Jiao, Tianhui
关键词:Antarctic krill meal; Cherax quadracarinatus; growth performance; fatty acid; immunity-related gene
-
Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii
作者:Su, Qiyao;Wei, Jie;Wang, Yakun;Liufu, Bai;Li, Huarong;Mai, Zhuang;Hong, Kunhao;Zhou, Qiaoyan;Jiao, Tianhui;Mu, Xidong;Yu, Lingyun;Su, Qiyao;Li, Huarong;Hong, Kunhao;Jiao, Tianhui;Liufu, Bai;Mai, Zhuang
关键词:
Macrobrachium rosenbergii ;myostatin ; RNAi; molt; growth -
17α-Methyltestosterone Affected Growth, Gonadal Development, and Intestinal Microbial Analysis in the Giant Freshwater Prawn (Macrobrachium rosenbergii)
作者:Bai, Liufu;Han, Zhiqiang;Bai, Liufu;Su, Qiyao;Hong, Kunhao;Wei, Jie;Wang, Yakun;Yu, Lingyun
关键词:17 alpha-methyltestosterone;
Macrobrachium rosenbergii ; gonadal development; histological analysis; sex ratio; intestinal flora -
Effects of Dietary Astaxanthin on Growth, Coloration, Immunity, and Antioxidant Capacity in Macrobrachium Rosenbergii
作者:Li, Huarong;Yu, Lingyun;Wei, Jie;Bai, Liufu;Su, Qiyao;Hong, Kunhao;Zhou, Qiaoyan;Zhu, Huaping;Wang, Yakun;Li, Huarong;Su, Qiyao;Hong, Kunhao;Bai, Liufu;Zhou, Qiaoyan
关键词:astaxanthin; body color; gene expression; growth performance; immunity; Macrobrachium rosenbergii
-
Toxic Effects of Povidone-Iodine on Macrobrachium rosenbergii: Concentration-Dependent Responses in Oxidative Stress, Immunosuppression, and Recovery Potential
作者:Jiao, Tianhui;Wang, Yakun;Wei, Jie;Xu, Sikai;Zhou, Qiaoyan;Mu, Xidong;Yu, Lingyun;Jiao, Tianhui
关键词:disinfectant; povidone-iodine; aquaculture industry;
Macrobrachium rosenbergii ; gills; mitochondrial apoptosis pathway; immunosuppression; restorative capacity; oxidative stress



