Effects of dietary glutathione on the growth performance, skin mucus antioxidant capacity, and immune responses of juvenile taimen Hucho taimen
文献类型： 外文期刊
作者： Wang, Chang'an 1 ; Lu, Shaoxia 1 ; Fan, Ze 1 ; Han, Shicheng 1 ; Liu, Yang 1 ; Jiang, Haibo 2 ; Liu, Hongbai 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Key Lab Aquat Anim Dis & Immune Technol Heilongji, 232 Hesong St, Harbin 150070, Peoples R China
2.Guizhou Univ, Coll Anim Sci, Guiyang 550025, Peoples R China
关键词： Glutathione; Feed additives; Skin inflammation; Gene expression; Hucho taimen
期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.953； 五年影响因子：2.94 ）
ISSN： 0967-6120
年卷期：
页码：
收录情况： SCI
摘要： A 49-day feeding trial was conducted to examine the effects of dietary glutathione on growth performance, skin antioxidant capacity, immune responses, and gene expression in juvenile taimen (Hucho taimen). The experimental fish had a mean initial weight of 5.36 +/- 0.13 g and were fed diets (approximately crude protein 460 g/kg, gross energy 22 MJ/kg) containing graded amounts of glutathione (0-control, 100, 200, 400, and 800 mg/kg). Each group had 3 replicates with 500 fish per tank (500 L). Fish were fed four times daily until evident satiation. After the feeding trial, 60 fish from each tank were challenged with lipopolysaccharide for 7 days, and the skin mucus antibacterial activity, antioxidant ability, and skin gene expression were also determined. The weight gain (WG, 3642.33-3843.67 g) and survival rate (SR, 93.07-94.19%) were all significantly improved in fish fed dietary glutathione (100-800 mg/kg) compared with the control group (WG, 3414.30 g; SR, 90.61%) (P < 0.05). After a 7-day lipopolysaccharide (3.00 mg/kg body weight) challenge, compared with the control group, dietary glutathione (100-800 mg/kg) significantly increased skin mucus protein, lysozyme activity (LZM, 2.67-2.74 U/mg; control, 2.11 U/mg), alkaline phosphate activity (ALP, 5.09-5.34 U/L; control, 4.50 U/L), minimal inhibitory concentration (MIC, 100-200 mu L/mL; control, > 200 mu L/mL), and antimicrobial activity (7.72-9.11 mm; control, 7.19 mm) (P < 0.05). Furthermore, dietary glutathione (100-800 mg/kg) also improved skin mucus antioxidant capacity and superoxide dismutase (SOD, 9.45-9.52 U/L; control, 7.51 U/L), and glutathione peroxidase (GSH-px, 9.68-10.11 U/L; control, 8.68 U/L) was significantly higher (P < 0.05), whereas the malondialdehyde (MDA, 0.18-0.20 nmol/L; control, 0.23 nmol/L) concentration was lower (P < 0.05). Additionally, dietary glutathione (100-800 mg/kg) significantly decreased the expression levels of skin tumor necrosis factor (TNF-alpha), interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), and interleukin 8 (IL-8), but increased the expression levels of interleukin 10 (IL-10) and nuclear factor-kappa-B inhibitor alpha-like (I kappa B alpha). In conclusion, dietary glutathione (100-800 mg/kg) improved the growth performance and decreased the lipopolysaccharide-induced skin inflammatory response, indicating that glutathione has the potential for anti-inflammatory effects on juvenile H. taimen.
- 相关文献
作者其他论文 更多>>
-
Comparative metabolomics analysis based on LC-MS method reveals regulatory difference in lipid metabolism and fatty acids metabolism between diploid and triploid rainbow trout (Oncorhynchus mykiss)
作者：Li, Chenhui;Chen, Zhongxiang;Qin, Dongli;Xu, Gefeng;Gao, Lei;Hao, Qirui;Du, Ningning;Fan, Ze;Sun, Yanchun;Wang, Peng;Li, Chenhui;Wang, Peng;Li, Chenhui;Wang, Peng;Li, Chenhui;Wang, Peng
关键词：Metabolites; Polyunsaturated fatty acid (PUFA); Triploid rainbow trout; Diploid rainbow trout
-
Genomic insights into the seawater adaptation in Cyprinidae
作者：Wang, Ying;Zhang, Xuejing;Xiong, Fei;Zhou, Min;Wang, Jing;Wang, Cheng;Qian, Yuting;Meng, Minghui;Chen, Wenjun;Ding, Zufa;Yu, Dan;Liu, Yang;He, Shunping;Yang, Liandong;Wang, Ying;He, Shunping;Yang, Liandong;Wang, Cheng;Qian, Yuting;Chen, Wenjun;Ding, Zufa;Yu, Dan;Liu, Yang;Chang, Yumei;Wang, Ying;Yang, Liandong
关键词：Far Eastern dace; Migratory; Osmoregulation; Seawater adaptation
-
Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm
作者：Ding, Xiaoyu;Tian, Yongsheng;Qiu, Yishu;Duan, Pengfei;Wang, Xinyi;Li, Zhentong;Li, Linlin;Liu, Yang;Wang, Linna;Tian, Yongsheng;Li, Zhentong;Li, Linlin;Liu, Yang;Wang, Linna;Tian, Yongsheng;Li, Zhentong;Li, Linlin;Liu, Yang;Wang, Linna
关键词：Epinephelus lanceolatus; sperm freezing damage; transcriptome analysis
-
Liver transcriptome and physiological analyses preliminarily revealed the adaptation mechanisms of Amur grayling (Thymallus arcticus grubei, Dybowski, 1869) fry for dietary lipid nutrition
作者：Fan, Ze;Ma, Kai;Wang, Yan;Wang, Liansheng;Zhang, Yongquan;Li, Chenhui;Li, Jiaxin;Wu, Di;Li, Jinnan;Wang, Yan;Li, Jiaxin;Li, Chenhui;Li, Zhengwei
关键词：Amur grayling; dietary lipid; growth performance; liver lipid metabolism; feedback regulation mechanism
-
Forest demography depends on stand structure, functional traits, and climate in the eastern region of the Qinghai-Tibet Plateau
作者：Li, Ting;Li, Ting;Tissue, David T.;Wright, Ian J.;Li, Ting;Wang, Qi;Wang, Qi;Wright, Ian J.;Luo, Peng;Luo, Peng;Lai, Changhong;Liu, Yang;Wang, Xiaodan;Wang, Xiaodan
关键词：Community dynamics; Forest demography; Growth; Mortality; Recruitment
-
Enhancing Growth and Intestinal Health in Triploid Rainbow Trout Fed a Low-Fish-Meal Diet through Supplementation with Clostridium butyricum
作者：Wang, Chang'an;Li, Fangyuan;Wang, Di;Lu, Shaoxia;Han, Shicheng;Gu, Wei;Liu, Hongbai;Wang, Chang'an;Jiang, Haibo;Li, Zhuang
关键词：triploid rainbow trout; C. butyricum; low-fish-meal diet; growth performance; intestinal health
-
Evaluation of four novel non-grain protein sources completely replacing soybean meal on growth performance, serum biochemistry, amino acid transport and intestinal health of grass carp ( Ctenopharyngodon idella) ) at different water temperatures
作者：Wu, Di;Fan, Ze;Zheng, Xianhu;Li, Jinnan;Wang, Liansheng;Zhou, Meng;Zhang, Haitao;Xu, Hong;Xie, Shouqi
关键词：Protein source; Temperature; Grass carp ( Ctenopharyngodon idella ); Growth performance; Protein utilization; Intestinal health
