文献类型： 外文期刊

作者： Lei, Rui-Feng ¹ ; Ma, Qin ¹ ; Li, Yu-Qian ² ; Abudourousuli, Dilireba ¹ ; Wang, Shuang ³ ; Rouzi, Zulihumaer ¹ ; Aosiman, Muyesaier ¹ ; An, Deng-Di ¹ ; Li, Wen-Jun ⁴ ;

作者机构： 1.Xinjiang Normal Univ, Coll Life Sci, Xinjiang Key Lab Special Species Conservat & Regu, Urumqi 830054, Peoples R China

2.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bio Resources & Ecol,Guan, Guangzhou 511458, Peoples R China

3.Heilongjiang Acad Agr Sci, Heilongjiang Acad Black Soil Conservat & Utilizat, Heilongjiang Fertilizer Engn Res Ctr, Key Lab Soil Environm & Plant Nutr Heilongjiang P, Harbin 150086, Peoples R China

4.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

5.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Peoples R China

6.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China

关键词： Bacillus suaedae sp. nov.; halophyte; haloalkaline ecosystem; Gurbantunggut desert; endophyte

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.689； 五年影响因子：2.925 ）

ISSN： 1466-5026

年卷期： 2022 年 72 卷 5 期

页码：

收录情况： SCI

摘要： YY A bacterial strain, designated YZJH907-2(T), was isolated from the stem of Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, Xinjiang, PR China. Cells of strain YZJH907-2(T) were Gram-stain-positive, aerobic and rod-shaped. They formed white or colourless circular colonies with smooth convex surfaces. Strain YZJH907-2(T) grew at 4-50 degrees C (optimum, 28-30 degrees C), pH 7.0-10.0 (optimum, pH 8.0-9.0) and with 0-10% (w/v) NaCl (optimum, 3-7 %). The genomic DNA G+C content of strain YZJH907-2(T) was 38.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the strain was most closely related to Bacillus alcalophilus DSM 485(T) (97.37%), Bacillus kiskunsagensis B16-24(T) (96.87%) and Bacillus bogoriensis LBB3(T) (96.71%). Average nucleotide identity values between YZJH907-2(T) and B. alcalophilus DSM 485(T) and B. bogoriensis LBB3(T) were 69.2 and 69.0 %, respectively. Digital DNA-DNA hybridization values of YZJH907-2(T) with B. alcalophilus DSM 485(T) and B. bogoriensis LBB3(T) were 19.6 and 20.4%, respectively. The cell wall of strain YZJH907-2(T) contained meso-diaminopimelic acid, and the major and secondary isoprenoid quinones were MK-7 and MK-5, respectively. Results of fatty acids showed that anteiso-C-15:0, iso-C-15:0 and C-16:0 were the predominant cellular fatty acids. Two-dimensional thin-layer chromatography analysis indicated that the polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. Based on the genomic, phylogenetic and phenotypic analyses, strain YZJH907-2(T) represented a novel species of the genus Bacillus, and thus the name Bacillus suaedae sp. nov. is proposed. The type strain is YZJH907-2(T) (=CGMCC 1.18763(T)=KCTC 43335(T)).