Root Transcriptome and Metabolome Profiling Reveal Key Phytohormone-Related Genes and Pathways Involved Clubroot Resistance in Brassica rapa L.
文献类型： 外文期刊
作者： Wei, Xiaochun 1 ; Zhang, Yingying 1 ; Zhao, Yanyan 1 ; Xie, Zhengqing 2 ; Hossain, Mohammad Rashed 3 ; Yang, Shuangjuan 1 ; Shi, Gongyao 2 ; Lv, Yanyan 1 ; Wang, Zhiyong 1 ; Tian, Baoming 2 ; Su, Henan 1 ; Wei, Fang 1 ; Zhang, Xiaowei 1 ; Yuan, Yuxiang 1 ;
作者机构： 1.Zhengzhou Univ, Henan Acad Agr Sci, Inst Hort, Grad T&R Base, Zhengzhou, Peoples R China
2.Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improve, Zhengzhou, Peoples R China
3.Bangladesh Agr Univ, Dept Genet & Plant Breeding, Mymensingh, Bangladesh
关键词： clubroot resistance; RNA-seq; phytohormone; metabolome profiling; Brassica rapa L; P; brassicae
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Plasmodiophora brassicae, an obligate biotrophic pathogen-causing clubroot disease, can seriously affect Brassica crops worldwide, especially Chinese cabbage. Understanding the transcriptome and metabolome profiling changes during the infection of P. brassicae will provide key insights in understanding the defense mechanism in Brassica crops. In this study, we estimated the phytohormones using targeted metabolome assays and transcriptomic changes using RNA sequencing (RNA-seq) in the roots of resistant (BrT24) and susceptible (Y510-9) plants at 0, 3, 9, and 20 days after inoculation (DAI) with P. brassicae. Differentially expressed genes (DEGs) in resistant vs. susceptible lines across different time points were identified. The weighted gene co-expression network analysis of the DEGs revealed six pathways including "Plant-pathogen interaction" and "Plant hormone signal transduction" and 15 hub genes including pathogenic type III effector avirulence factor gene (RIN4) and auxin-responsive protein (IAA16) to be involved in plants immune response. Inhibition of Indoleacetic acid, cytokinin, jasmonate acid, and salicylic acid contents and changes in related gene expression in R-line may play important roles in regulation of clubroot resistance (CR). Based on the combined metabolome profiling and hormone-related transcriptomic responses, we propose a general model of hormone-mediated defense mechanism. This study definitely enhances our current understanding and paves the way for improving CR in Brassica rapa.
- 相关文献
作者其他论文 更多>>
-
Formation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber Fusarium Wilt
作者：Yang, Fan;Wang, Xin;Jiang, Huayan;Chang, Xiaoke;Yao, Qiuju;Wang, Xin;Jiang, Huayan;Chen, Weiwei;Shi, Gongyao;Tian, Baoming
关键词：synthetic microbial community; single-factor test; orthogonal test; response surface method; cucumber
Fusarium wilt -
Influence of Soil Physicochemical Properties and Inter-Root Microbial Communities on the Inhibition of Anthracnose in Peppers
作者：Ma, Yongbin;Wang, Yichao;Xiao, Huaijuan;Liu, Miaomiao;Hong, Yuting;Shi, Gongyao;Liu, Miaomiao;Hong, Yuting;Chang, Xiaoke;Yao, Qiuju;Yang, Fan
关键词：pepper; anthracnose; soil properties; high-throughput sequencing; rhizosphere microbial communities
-
A chromosome-level reference genome facilitates the discovery of clubroot-resistant gene Crr5 in Chinese cabbage
作者：Yang, Shuangjuan;Zhang, Wenjing;Su, Henan;Wei, Xiaochun;Zhao, Yanyan;Wang, Zhiyong;Zhang, Xiaowei;Yuan, Yuxiang;Wang, Xiangfeng;Guo, Li;Wang, Zhaojun
关键词：
-
Transcriptome Analysis Revealed Hub Genes Related to Tipburn Resistance in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
作者：Bi, Yaning;Zhang, Wenjing;Yuan, Yuxiang;Zhao, Yanyan;Li, Lin;Su, Henan;Tian, Baoming;Wei, Fang;Wei, Xiaochun;Zhang, Xiaowei;Bi, Yaning;Tian, Baoming;Wei, Fang;Wei, Xiaochun;Feng, Jianqi;Wang, Peiyun;Ding, Cong
关键词：tipburn; Chinese cabbage; calcium deficiency; RNA-seq
-
Differential Sensitivity of Photosynthetic Electron Transport to Dark-Induced Senescence in Wheat Flag Leaves
作者：Yang, Cheng;Du, Simeng;Shi, Yanhua;Zhang, Deqi;Yue, Junqin;Li, Xiangdong;Jin, Haiyang;Fang, Baoting;Yan, Ge;Wei, Fang;Yan, Ge;Zhang, Zishan
关键词：Wheat; Senescence; Prompt fluorescence; Modulated 820-nm reflection; Delayed fluorescence
-
Chromosome pairing during meiosis in Brassica hybrid allodiploids with evolutionarily related genomes
作者：Li, Xiaoying;Liu, Yue;Xie, Zhengqing;Tian, Baoming;Datta, Tanusree;Wei, Fang;Shi, Gongyao;Yuan, Yuxiang;Wei, Xiaochun
关键词：
Brassica allodiploid; Meiosis; Chromosome pairing; Synapsis; Transcriptome; Homologous recombination -
Mitotic pollen abnormalities are linked to Ogura cytoplasmic male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
作者：Wei, Xiaochun;Zhang, Yuanlin;Zhao, Yanyan;Yang, Shuangjuan;Su, Henan;Wang, Zhiyong;Zhang, Wenjing;Wei, Fang;Yuan, Yuxiang;Zhang, Xiaowei;Wei, Xiaochun;Zhang, Yuanlin;Chen, Weiwei;Tian, Baoming;Wei, Fang;Zhang, Xiaowei;Nath, Ujjal Kumar
关键词：Ogura CMS; Chinese cabbage; cytology; transcriptome; PCD; pollen abortion
