Droplet digital polymerase chain reaction (ddPCR) for rapid screening of adulterants in honey: A case study on acacia honey adulterated with canola honey
文献类型： 外文期刊
作者： You, Zhaotong 1 ; Mei, Yingting 3 ; Wang, Xiaofu 1 ; Chen, Xiaoyun 1 ; Xu, Junfeng 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
2.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China
3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 10081, Peoples R China
关键词： Honey; ddPCR; Adulteration; DNA extraction; Pollen
期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）
ISSN： 0956-7135
年卷期： 2021 年 130 卷
页码：
收录情况： SCI
摘要： In this study, droplet digital polymerase chain reaction (ddPCR) was used to detect adulteration of acacia honey with canola honey. DNA extraction from pollen in acacia honey and canola honey was performed using four different pollen treatment methods, including, enzymatic hydrolysis, freeze-thaw, mechanical, and ultrasonic. The mechanical extraction method was the most preferred method of DNA extraction from honey. A duplex ddPCR method developed based on the specific target gene in acacia and canola was performed. The detection results showed that the established duplex ddPCR had a high detection sensitivity, detecting up to 1% adulteration. The accuracy of duplex ddPCR was consistent with the traditional melissopalynologic method. All results indicated that ddPCR was a powerful method for assessing the authenticity and quality of honey.
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