Identification of sex-specific DNA markers: Providing molecular evidence for the ZW sex determination system in the redclaw crayfish (Cherax quadricarinatus)
文献类型： 外文期刊
作者： Jin, Ling 1 ; Jia, Shaoting 1 ; Zhang, Wen 1 ; Chen, Yibin 3 ; Li, Sedong 3 ; Liu, Ping 1 ; Li, Jian 1 ; Lv, Jianjian 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao, Peoples R China
3.Guangdong Evergreen Feed Ind Co Ltd, Evergreen Tower,23 Leshan Rd, Zhanjiang City, Guangdong, Peoples R China
4.Zhanjiang Ocean & Fishery Dev Res Ctr, Zhanjiang 524000, Peoples R China
关键词： Cherax quadricarinatus; Next-generation sequencing; W chromosome; Sex-specific marker
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 546 卷
页码：
收录情况： SCI
摘要： The redclaw crayfish (Cherax quadricarinatus) exhibit sex dimorphism, and thus, the discovery of sex-specific markers is of great significance for the study of monosexual breeding and sex-determination mechanisms in this species. In this study, sex-specific markers were found using a next-generation sequencing (NGS)-based approach. A total of 482.7 Gb clean data were obtained, which included 1,568,549,460 and 1,625,511,910 Paired-end (PE) reads from the female and male libraries, respectively. After filtering out the contigs that could be aligned to the male reads, 94 candidate W chromosome-specific contigs were identified. Among these, 12 contigs were selected and verified in different populations. Finally, five sex-specific markers were developed. Additionally, a pair of primers of Z/W-76567 was developed as a co-dominant marker. Electrophoretic analysis of the Z/W-76567 PCR product showed clear double bands for females and a single band for males. Our results provide molecular evidence for the ZW sex chromosome system in this species, which has tremendous potential for clarifying the mechanism of sex determination in C. quadricarinatus.
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