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OsTKPR1 proteins with a single amino acid substitution fail the synthesis of a specific sporopollenin precursor and cause abnormal exine and pollen development in rice

文献类型： 外文期刊

作者： Liu, Feng 1 ; Yang, Huiting 1 ; Tang, Rong 1 ; Wang, Wang 1 ; Shen, Haodong 1 ; Xu, Mengxue 1 ; Hao, Tiancheng 1 ; Hu, Yuanyuan 1 ; Zhang, Yunhui 2 ; Bao, Yiqun 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Prov Key Lab Agrobiol, Nanjing, Peoples R China

关键词： OsTKPR1(P124S); Pollen exine; Reduced tetraketide & alpha;-pyrone; Rice; Sporopollenin

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 335 卷

页码：

收录情况： SCI

摘要： Fatty acid derivatives are key components of rice pollen exine. The synthesis of aliphatic sporopollenin precursors are initiated in the plastids of the tapetal cells, followed by multiple-step reactions conducted in the endoplasmic reticulum (ER). However, the relative contribution of different precursors to the precise structure of sporopollenin remains largely elusive, let alone the underlying mechanism. Here, we report that two complete male sterile mutants ostkpr1-3 (Tetraketide a-pyrone reductase 1-3, with OsTKPR1(P124S) substitution) and ostkpr1-4 (with truncated OsTKPR1(stop)) are defective in pollen exine, Ubisch body and anther cuticle development where ostkpr1-4 display severer phenotypes. Remarkably, OsTKPR1 could produce reduced hydroxylated tetraketide a-pyrone and reduced tetraketide a-pyrone, whereas OsTKPR1(P124S) fails to produce the latter. Pairwise interaction assays show that mutated OsTKPR1(P124S) is able to integrate into a recently characterized metabolon, thus its altered catalytic activity is not due to dis-integrity of the metabolon. In short, we find that reduced tetraketide a-pyrone is a key sporopollenin precursor required for normal exine formation, and the conserved 124th proline of OsTKPR1 is essential for the reduction activity. Therefore, this study provided new insights into the sporopollenin precursor constitution critical for exine formation.

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