文献类型： 外文期刊

作者： Haider, Muhammad Salman ¹ ; Sheikh, Taha Majid Mahmood ² ; Jiu, Songtao ³ ; Aleem, Muqaddas ⁴ ; Shafqat, Waqar ⁵ ; Shoukat, Komal ⁶ ; Khan, Nadeem ⁷ ; Jaskani, Muhammad Jafar ⁸ ; Naqvi, Summar A. ⁸ ; Ercisli, Sezai ⁹ ; Assouguem, Amine ¹⁰ ; Kara, Mohammed ¹¹ ; Ullah, Riaz ¹² ; Aljabri, Maha ¹³ ; Qari, Sameer H. ¹⁴ ;

作者机构： 1.Ghazi Univ, Dept Hort, Dera Ghazi Khan 32200, Pakistan

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

3.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Plant Sci, Shanghai 200240, Peoples R China

4.Univ Agr Faisalabad, Ctr Adv Studies Agr & Food Secur, Faisalabad 38040, Pakistan

5.Mississippi State Univ, Coll Forest Resources, Dept Forestry, Starkville, MI 39759 USA

6.Govt Coll Women Univ, Dept Environm Sci, Sailkot 51310, Pakistan

7.Univ Ottawa, Dept Biol, 30 Marie Curie, Ottawa, ON K1N 6N5, Canada

8.Univ Agr Faisalabad, Inst Hort Sci, Faisalabad 38040, Pakistan

9.Ataturk Univ, Fac Agr, Dept Hort, TR-25240 Erzurum, Turkey

10.Sidi Mohamed Ben Abdellah Univ, Fac Sci & Technol, Lab Funct Ecol & Environm, Imouzzer St, Fes 30000, Morocco

11.Sidi Mohamed Ben Abdellah Univ, Lab Biotechnol & Conservat & Valorizat Nat Resour, Fes 30000, Morocco

12.King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia

13.Umm Al Qura Univ, Fac Appl Sci, Res Labs Ctr, Dept Biol, Mecca 24382, Saudi Arabia

14.Umm Al Qura Univ, Al Jumum Univ Coll, Dept Biol, Mecca 24382, Saudi Arabia

关键词： strawberry; cation diffusion facilitator; metal tolerance proteins; cadmium stress

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 6 期

页码：

收录情况： SCI

摘要： Metal tolerance proteins (MTPs) are divalent cation transporters, known to upkeep the mineral nutrition of plants and heavy metal transport at cell, tissue, or whole plant levels. However, information related to evolutionary relationships and biological functions of MTP genes in strawberry (Fragaria vesca L.) remain elusive. Herein, we identified 12 MTP genes from the strawberry genome and divided them into three main groups (i.e., Zn-MTP, Fe/Zn MTP, and Mn-MTP), which is similar to MTP grouping in Arabidopsis and rice. The strawberry MTPs (FvMTPs) are predicted to be localized in the vacuole, while open reading frame (ORF) length ranged from 1113 to 2589 bp with 370 to 862 amino acids, and possess 4 to 6 transmembrane domains (TMDs), except for FvMTP12 that possessed 16 TMDs. All the FvMTP genes had putative cation efflux and cation diffusion facilitator domains along with a zinc dimerization (ZT-dimer) domain in Mn-MTPs. The collinear analysis suggested their conservation between strawberry and Arabidopsis MTPs. Promoter analysis also demonstrated that some of them might possibly be regulated by hormones and abiotic stress factors. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis proposed that FvMTP genes are involved in cation transport and homeostasis. The expression analysis showed that FvMTP1, FvMTP1.1, and FvMTP4 were significantly induced in leaf samples, while FvMTP1.1 and FvMTP4 were significantly regulated in roots of cadmium (Cd)-treated strawberry plants during progressive stress duration. The findings of Cd accumulation depicted that Cd contents were significantly higher in root tissues than that of leaf tissues of strawberry. These results are indicative of their response during the specific duration in Cd detoxification, while further functional studies can accurately verify their specific role.