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Microwave-assisted degradation of beta-D-glucan from Ganoderma lucidum and the structural and immunoregulatory properties of oligosaccharide fractions

文献类型： 外文期刊

作者： Qin, Xiu 1 ; Ma, Guanhua 1 ; Liu, Liping 1 ; Feng, Jie 1 ; Zhou, Shuai 1 ; Han, Wei 2 ; Zhou, Jing 1 ; Liu, Yanfang 1 ; Zhang, Jingsong 1 ;

作者机构： 1.Natl Engn Res Ctr Edible Fungi, Shanghai Acad Agr Sci, Inst Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South,Mi, Shanghai 201403, Peoples R China

2.East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Shanghai Key Lab New Drug Design,Minist Educ, Shanghai 200237, Peoples R China

3.Shanghai Baixin Biotech Co Ltd, Shanghai 201403, Peoples R China

关键词： beta-Glucooligosaccharide; HPAEC-PAD-MS/MS; Dectin-1

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 220 卷

页码：

收录情况： SCI

摘要： Microwave-assisted degradation of beta-(1 -> 3,1 -> 6)-D-glucan from Ganoderma lucidum and correlated immunoregulatory activities were investigated in this study. The optimal temperature and degradation time for microwave hydrothermal hydrolysis were 140 degrees C and 40 min, respectively. Under these conditions, a high yield of degradation rate (98.4 %) and abundant beta-oligosaccharide products (GLOS) with different degrees of polymerization (DP 2-24) were obtained. Four fractions including F1 (DP 2-8), F2 (DP 6-19), F3 (DP 8-24) and F4 (high DPs) with different average ratios of beta-(1 -> 3) to beta-(1 -> 6)-linked glucose units were isolated from GLOS. The structures of oligosaccharides with DP (2-6) in Fl were identified as linear beta-(1 -> 3)-linked glucooligosaccharides without or with beta-(1 -> 6)-linked glucose residues based on MS/MS analysis. The immunoregulation activity of beta-glucooligosaccharides was correlated with their DPs and the average ratios of beta-(1 -> 3) to beta-(1 -> 6)- linked glucose units. F4 fraction with high DPs and ratio of 3.29:1 exhibited higher immunoenhancing activity on inducing NF-kappa B activation through binding to dectin-1. Surface plasmon resonance (SPR) analysis indicated that beta-glucooligosaccharides could bind to Dectin-1 directly and the binding affinity increased with the increase of DPs and the ratios of beta-(1 -> 3)-linked glucose.

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