Effects of Antibiotic Residues on Fecal Microbiota Composition and Antimicrobial Resistance Gene Profiles in Cattle from Northwestern China
文献类型： 外文期刊
作者： He, Wei 1 ; Wang, Xiaoming 1 ; Cao, Yuying 1 ; Liu, Cong 1 ; Qin, Zihui 1 ; Zuo, Yang 3 ; Li, Yiming 4 ; Tang, Fang 1 ; Dai, Jianjun 1 ; Wang, Shaolin 3 ; Xue, Feng 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
2.Ningxia Hui Autonomous Reg Food Testing & Res Inst, Yinchuan 750002, Peoples R China
3.China Agr Univ, Coll Vet Med, Natl Key Lab Vet Publ Hlth & Safety, Beijing 100193, Peoples R China
4.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
5.China Pharmaceut Univ, Sch Engn, Nanjing 210009, Peoples R China
6.Minist Agr & Rural Affairs, Key Lab Anim Antimicrobial Resistance Surveillance, Beijing 100081, Peoples R China
7.Sanya Inst China Agr Univ, Technol Innovat Ctr Food Safety Surveillance & Det, Sanya 572025, Peoples R China
关键词： grazing cattle; antibiotic residues; microbial communities; antibiotic resistance genes
期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN：
年卷期： 2025 年 13 卷 7 期
页码：
收录情况： SCI
摘要： Grazing is a free-range farming model commonly practiced in low-external-input agricultural systems. The widespread use of veterinary antibiotics in livestock farming has led to significant environmental accumulation of antibiotic residues and antibiotic resistance genes (ARGs), posing global health risks. This study investigated the antibiotic residues, bacterial community, ARG profiles, and mobile genetic elements (MGEs) in cattle feces from three provinces in western China (Ningxia, Xinjiang, and Inner Mongolia) under grazing modes. The HPLC-MS detection showed that the concentration of tetracycline antibiotics was the highest in all three provinces. Correlation analysis revealed a significant negative correlation between antibiotic residues and the diversity and population abundance of intestinal microbiota. However, the abundance of ARGs was directly proportional to antibiotic residues. Then, the Sankey analysis revealed that the ARGs in the cattle fecal samples were concentrated in 15 human pathogenic bacteria (HPB) species, with 9 of these species harboring multiple drug resistance genes. Metagenomic sequencing revealed that carbapenemase-resistant genes (blaKPC and blaVIM) were also present in considerable abundance, accounting for about 10% of the total ARGs detected in three provinces. Notably, Klebsiella pneumoniae strains carrying blaCTX-M-55 were detected, which had a possibility of IncFII plasmids harboring transposons and IS19, indicating the risk of horizontal transfer of ARGs. This study significantly advances the understanding of the impact of antibiotic residues on the fecal microbiota composition and ARG profiles in grazing cattle from northwestern China. Furthermore, it provides critical insights for the development of rational antibiotic usage strategies and comprehensive public health risk assessments.
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