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Arsenic inhibits citric acid accumulation via downregulating vacuolar proton pump gene expression in citrus fruits

文献类型： 外文期刊

作者： Qiu, Diyang 1 ; Zhu, Congyi 1 ; Fan, Ruiyi 1 ; Mao, Genlin 1 ; Wu, Pingzhi 1 ; Zeng, Jiwu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangdong Prov Key Lab Trop & Subtrop Fruit Tree R, Guangzhou 510640, Peoples R China

2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, 80 Dafeng 2nd St,Tianhe, Guangzhou 510640, Peoples R China

关键词： Citrus spp; (citrus); Citric acid; Arsenic; Proton pump; Oxidative stress; Mitochondria

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 246 卷

页码：

收录情况： SCI

摘要： Citric acid content is a critical quality determinant in citrus (Citrus spp.) fruits. Although arsenic (As) can effectively reduce citric acid content to improve citrus fruit quality, it can have adverse environmental effects. The discovery of nontoxic substitutes is hampered by the incomplete elucidation of the underlying mechanisms of As action in citrus fruits. Metabolic, transcriptomic, and physiological analyses were employed to investigate As action on citric acid accumulation to discover the mechanisms of As action in citrus. The enzyme activity related to citrate biosynthesis was not inhibited and the content of the involved metabolites was not reduced in As-treated fruits. However, the proton pump genes CitPH5 and CitPH1 control the vacuolar citric acid accu-mulation and transcription factor genes CitTT8 and CitMYB5, which regulate CitPH5 and CitPH1, were down -regulated. The oxidative stress-response genes were upregulated in As-treated fruits. The reactive oxygen species (ROS) treatment also downregulated CitTT8 and CitMYB5 in juice cells. The mitochondrial ROS production rate increased in As-treated fruits. AsIII was more potent in stimulating isolated mitochondria to overproduce ROS compared to AsV. Our results indicate that the As inhibition of citric acid accumulation may be primarily due to the transcriptional downregulation of CitPH5, CitPH1, CitTT8, and CitMYB5. As-induced oxidative stress signaling may operate upstream to downregulate these acid regulator genes. Mitochondrial thiol proteins may be the principal targets of As action in citrus fruits.

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