文献类型： 外文期刊
作者： Wang, Ying 1 ; Chen, Hongyu 1 ; Ma, Liang 2 ; Gong, Ming 1 ; Wu, Yingying 1 ; Bao, Dapeng 1 ; Zou, Gen 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China
2.Zhejiang A&F Univ, Coll Adv Agr Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Linan Hangzhou, Peoples R China
期刊名称：MICROBIAL BIOTECHNOLOGY （ 影响因子：6.575； 五年影响因子：7.412 ）
ISSN： 1751-7915
年卷期：
页码：
收录情况： SCI
摘要： Given their lignocellulose degradability and biocontrol activities, fungi of the ubiquitously distributed genus Trichoderma have multiple industrial and agricultural applications. Genetic manipulation plays a valuable role in tailoring novel engineered strains with enhanced target traits. Nevertheless, as applied to fungi, the classic tools of genetic manipulation tend to be time-consuming and tedious. However, the recent development of the CRISPR-Cas system for gene editing has enabled researchers to achieve genome-wide gene disruptions, gene replacements, and precise editing, and this technology has emerged as a primary focus for novel developments in engineered strains of Trichoderma. Here, we provide a brief overview of the traditional approaches to genetic manipulation, the different strategies employed in establishing CRSIPR-Cas systems, the utilization of these systems to develop engineered strains of Trichoderma for desired applications, and the future trends in biotechnology.
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