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Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells

文献类型： 外文期刊

作者： Wang, Yue 1 ; Wang, Hui-Li 1 ; Lin, Zhi-Ping 3 ; Zhong, Ji-Feng 1 ; Chen, Kun-Lin 1 ; Duan, Xing 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Inst Anim Sci, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China

2.Zhejiang A&F Univ, Coll Vet Med, Coll Anim Sci & Technol, Key Lab Appl Technol Green Ecohlth Anim Husb Zhej, Hangzhou 311300, Peoples R China

3.Youyuan Res Inst Dairy Ind Co Ltd, Nanjing 211100, Peoples R China

关键词： Exosomes; Bovine mammary epithelial cells; Heat stress; Mitochondrial function; Milk fat; Milk protein

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.742； 五年影响因子：2.702 ）

ISSN： 0301-4851

年卷期： 2022 年 49 卷 10 期

页码：

收录情况： SCI

摘要： Background Exosomes are involved in intercellular communication, affecting many physiological and pathological process. The present study evaluated the effects of serum exosomes on the function of bovine mammary epithelial cells (BMECs) and milk synthesis under heat stress. Methods and results We cultured the BMECs in fetal bovine serum (FBS) or exosome-free FBS medium and examined, their viability using CCK-8 kit. The results showed that culturing the cells in an exosome-free medium decreased viability and increased the levels of reactive oxygen species. The BMECs cultured in the exosome-free medium had reduced mitochondrial membrane potential, decreased manganese superoxide dismutase activity, and disrupted mitochondrial dynamics. They exhibited apoptosis due to upregulated Drp1, Fis1, Bax and HSP70. Lastly, we observed downregulation of milk fat and lactoprotein-related genes: mTOR, PPAR gamma, p-mTOR and ADD1 and SREBP1, ELF5, and CSN2, respectively, after culturing the cells in an exosome-free medium. These negative effects of the exosome-free medium on the BMECs could be further reinforced under heat stress. Conclusion Our results demonstrated that exosomes from serum are critical for maintaining the normal function of BMECs.

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