Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
文献类型: 外文期刊
作者: Ding, Qiang 1 ; Jin, Miaohan 2 ; Kalds, Peter 2 ; Meng, Chunhua 1 ; Wang, Huili 1 ; Zhong, Jifeng 1 ; Wang, Xiaolong 2 ;
作者机构: 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
2.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Xianyang 712100, Peoples R China
3.Arish Univ, Fac Environm Agr Sci, Dept Anim & Poultry Prod, Al Arish 45511, Egypt
关键词: extracellular vesicles; follicular fluid; small RNA sequence; miRNAs
期刊名称:ANIMALS ( 影响因子:2.752; 五年影响因子:2.942 )
ISSN: 2076-2615
年卷期: 2021 年 11 卷 11 期
页码:
收录情况: SCI
摘要: Simple Summary:& nbsp;Ovarian follicular development is associated with ovulation and is further related to litter size in goats. Extracellular vesicles (EVs) derived from miRNAs within follicular fluid undergo dynamic changes, and, together with follicle growth, may be considered as potential regulators of follicular development. However, the function and changes in EVs remain ambiguous. Here, we identified miRNA changes in EVs from small to large goat follicular fluid. Using bioinformatics tools, we demonstrated the existence of differentially expressed miRNAs in EVs from follicles of different sizes that are responsible for an altered biological effect. This study contributes to a better understanding of follicular development in goats.& nbsp;Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.
- 相关文献
作者其他论文 更多>>
-
Effect of Garlic Straw with Silage Corn Stalks on Hu Sheep Rumen Fermentation and Microbial Community In Vitro
作者:Shen, Yangyang;Zhang, Jianli;Gui, Hongbing;Wang, Huili;Li, Yinxia;Zhang, Jun;Cao, Shaoxian;Zhong, Jifeng;Qian, Yong;Meng, Chunhua;Shen, Yangyang;Zhang, Jianli;Gui, Hongbing;Wang, Huili;Li, Yinxia;Zhang, Jun;Cao, Shaoxian;Zhong, Jifeng;Qian, Yong;Meng, Chunhua;Shen, Yangyang;Zhang, Jianli;Wang, Huili;Li, Yinxia;Zhang, Jun;Cao, Shaoxian;Zhong, Jifeng;Qian, Yong;Meng, Chunhua;Gui, Hongbing
关键词:garlic straw; Hu sheep digestibility; ruminal fermentation; volatile fatty acids; microbiota
-
miR-27a-3p targets NR5A2 to regulate CYP19A1 expression and 17-beta estradiol synthesis in ovine granulosa cells
作者:Gui, Hongbing;Li, Fan;Chen, Cheng;Zhu, Qiuyi;Zhang, Chenjian;Zhang, Jun;Meng, Chunhua;Qian, Yong;Cao, Shaoxian;Li, Yinxia;Gui, Hongbing;Li, Fan;Zhang, Jun;Meng, Chunhua;Qian, Yong;Cao, Shaoxian;Li, Yinxia;Gui, Hongbing;Li, Fan;Zhang, Jun;Meng, Chunhua;Qian, Yong;Cao, Shaoxian;Li, Yinxia
关键词:MiR-27a-3p; Granulosa cells; Estradiol synthesis; Hu sheep
-
Enhancing energy efficiency and reducing carbon footprint in organic soybean production through no-tillage and rye cover crop integration
作者:Huang, Qiliang;Dewi, Ratih Kemala;Hashimi, Rahmatullah;Gong, Yingting;Dewi, Ratih Kemala;Li, Peiran;Wang, Xiaolong;Hashimi, Rahmatullah;Komatsuzaki, Masakazu
关键词:Organic farming; No-tillage; Cover crop; Energy budgeting; Carbon footprint
-
Efficient and Specific Generation of MSTN-Edited Hu Sheep Using C-CRISPR
作者:Guo, Rihong;Wang, Huili;Meng, Chunhua;Gui, Hongbing;Li, Yinxia;Chen, Fang;Zhang, Chenjian;Zhang, Han;Ding, Qiang;Zhang, Jianli;Zhang, Jun;Qian, Yong;Zhong, Jifeng;Cao, Shaoxian;Guo, Rihong;Wang, Huili;Meng, Chunhua;Li, Yinxia;Chen, Fang;Ding, Qiang;Zhang, Jianli;Zhang, Jun;Qian, Yong;Zhong, Jifeng;Cao, Shaoxian;Cao, Shaoxian
关键词:CRISPR; Cas9; Hu sheep; MSTN; double muscling phenotype; off-target effects
-
Exposure to diisononyl phthalate deteriorates the quality of porcine oocytes by inducing the apoptosis
作者:Wang, Rui;Chen, Jingyue;Cui, Zhaokang;Li, Yu;Miao, Yilong;Xiong, Bo;Chen, Jingyue;Cui, Zhaokang;Wang, Huili;Gao, Qian
关键词:Diisononyl phthalate; Oocyte quality; Fertilization ability; Apoptosis; Autophagy
-
miR-27a-3p relieves heat stress-induced mitochondrial damage and aberrant milk protein synthesis through MEK/ERK pathway in BMECs
作者:Wang, Yue;Xia, Shu-Wen;Zhao, Fang;Ding, Qiang;Ye, Xiao-Mei;Zhong, Ji-Feng;Chen, Kun-Lin;Wang, Hui-Li;Wang, Yue;Xia, Shu-Wen;Zhao, Fang;Ding, Qiang;Ye, Xiao-Mei;Zhong, Ji-Feng;Chen, Kun-Lin;Wang, Hui-Li;Wu, Jie
关键词:miR-27a-3p; Mitochondrial damage; Cell proliferation; Lactation; MEK; ERK signaling pathway
-
SIRT4 Expression Ameliorates the Detrimental Effect of Heat Stress via AMPK/mTOR Signaling Pathway in BMECs
作者:Ding, Qiang;Wang, Yue;Xia, Shu-Wen;Zhao, Fang;Zhong, Ji-Feng;Wang, Hui-Li;Chen, Kun-Lin
关键词:SIRT4; heat stress; lactation; mitochondrial function; AMPK; mTOR signaling pathway