您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

文献类型： 外文期刊

作者： Ding, Qiang 1 ; Jin, Miaohan 2 ; Kalds, Peter 2 ; Meng, Chunhua 1 ; Wang, Huili 1 ; Zhong, Jifeng 1 ; Wang, Xiaolong 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China

2.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Xianyang 712100, Peoples R China

3.Arish Univ, Fac Environm Agr Sci, Dept Anim & Poultry Prod, Al Arish 45511, Egypt

关键词： extracellular vesicles; follicular fluid; small RNA sequence; miRNAs

期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）

ISSN： 2076-2615

年卷期： 2021 年 11 卷 11 期

页码：

收录情况： SCI

摘要： Simple Summary:& nbsp;Ovarian follicular development is associated with ovulation and is further related to litter size in goats. Extracellular vesicles (EVs) derived from miRNAs within follicular fluid undergo dynamic changes, and, together with follicle growth, may be considered as potential regulators of follicular development. However, the function and changes in EVs remain ambiguous. Here, we identified miRNA changes in EVs from small to large goat follicular fluid. Using bioinformatics tools, we demonstrated the existence of differentially expressed miRNAs in EVs from follicles of different sizes that are responsible for an altered biological effect. This study contributes to a better understanding of follicular development in goats.& nbsp;Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台