miR-27a-3p targets NR5A2 to regulate CYP19A1 expression and 17-beta estradiol synthesis in ovine granulosa cells
文献类型： 外文期刊
作者： Gui, Hongbing 1 ; Li, Fan 1 ; Chen, Cheng 1 ; Zhu, Qiuyi 1 ; Zhang, Chenjian 1 ; Zhang, Jun 1 ; Meng, Chunhua 1 ; Qian, Yong 1 ; Cao, Shaoxian 1 ; Li, Yinxia 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
2.Jiangsu Prov Platform Conservat & Utilizat Agr Ger, Nanjing 210014, Peoples R China
3.Minist Agr, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China
关键词： MiR-27a-3p; Granulosa cells; Estradiol synthesis; Hu sheep
期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.2； 五年影响因子：2.3 ）
ISSN： 0378-4320
年卷期： 2023 年 248 卷
页码：
收录情况： SCI
摘要： Although 17-beta estradiol (E2) synthesis is important in regulating female fertility, we know little regarding the molecular mechanism of miRNA-regulated ovine E2 synthesis. Here, our experiments with granulosa cells (GCs) from Hu sheep revealed miR-27a-3p involvement in E2 synthesis and its association with ovine litter size. First, we showed that miR-27a-3p of sheep and other mammals share a high nucleotide identity. Next, gain- and loss-of-function assays indicated that miR-27a-3p inhibits CYP19A1 expression and E2 synthesis in GCs. Moreover, we demonstrated that NR5A2 is a direct target of miR-27a-3p. Ovine miR-27a-3p suppresses E2 synthesis via the NR5A2 and CYP19A1 axes. We also identified four single nucleotide polymorphisms in the ovine miR-27a gene, and g.-13 G>A and g 0.24 T > G were significantly associated with the first and the second parity litter size, respectively (P < 0.05). In summary, our findings reveal that miR27a-3p is a novel regulator of E2 synthesis and may predict litter size of Hu sheep, providing insight into mechanisms underlying granulosa cell function and female fertility.
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