Engineering an enzyme-like catalytic sensor for on-site dual-mode evaluation of total antioxidant capacity
文献类型： 外文期刊
作者： Han, Xi mei 1 ; Dan, Jie 1 ; Chen, Hao qian 1 ; Wang, Qian 1 ; Luo, Lin pin 1 ; Feng, Jian xing 1 ; Wang, Tian yu 1 ; Sun, Jian 3 ; Wang, Jian long 1 ; Gu, Ying 2 ; Zhang, Wentao 1 ;
作者机构： 1.Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
2.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China
3.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun 130000, Peoples R China
关键词： Fe-MoOx; POD-like activity; Colorimetric assay; Total antioxidant capacity (TAC); Dual-mode detection
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：5.0 ）
ISSN： 0026-3672
年卷期： 2024 年 191 卷 8 期
页码：
收录情况： SCI
摘要： A novel Fe-MoOx nanozyme, engineered with enhanced peroxidase (POD)-like activity through strategic doping and the creation of oxygen vacancies, is introduced to catalyze the oxidation of TMB with high efficiency. Furthermore, Fe-MoOx is responsive to single electron transfer (SET) and hydrogen atom transfer (HAT) mechanisms related to antioxidants and can serve as a desirable nanozyme for total antioxidant capacity (TAC) determination. The TAC colorimetric platform can reach a low LOD of 0.512 mu M in solution and 24.316 mu M in the smartphone-mediated RGB hydrogel (AA as the standard). As proof of concept, the practical application in real samples was explored. The work paves a promising avenue to design diverse nanozymes for visual on-site inspection of food quality.
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