FRET with MoS2 nanosheets integrated CRISPR/Cas12a sensors for robust and visual food-born parasites detection
文献类型： 外文期刊
作者： Chen, Xiuqin 1 ; Liu, Xiaolei 1 ; Yu, Yao 1 ; Wang, Haolu 1 ; Li, Chengyao 1 ; Vallee, Isabelle 4 ; Liu, Mingyuan 1 ; Zhao, Lianjing 1 ; Bai, Xue 1 ;
作者机构： 1.Jilin Univ, Inst Zoonosis, State Key Lab Diag & Treatment Severe Zoonot Infec, Key Lab Zoonosis Res,Minist Educ, Changchun 130062, Peoples R China
2.Jilin Univ, Coll Vet Med, Changchun 130062, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China
4.INRAE, ANSES, Ecole Natl Vet Alfort, UMR BIPAR,Lab Sante Anim, Maisons Alfort, France
5.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225000, Peoples R China
关键词： CRISPR/Cas12a; MoS2 nanosheets; Forster resonance energy transfer; Point-of-care testing; Parasite detection
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）
ISSN：
年卷期： 2023 年 395 卷
页码：
收录情况： SCI
摘要： Recently, CRISPR/Cas associated biosensors have been shown to have great potential in sensing applications due to their high sensitivity and high base resolution. However, the signal reporter system containing two organic fluorescent dye pair is limited by high cost and less stability. In contrast, functional nanomaterials exhibit robust stability, excellent optical properties and low preparation cost, making them suitable reporters. In this study, a MoS2 nanosheets (NSs) improved CRISPR/Cas12a-based biosensing platform was constructed for the first time to detect food-borne parasites. MoS2 NSs were used as fluorescence quenchers and single-stranded DNA (ssDNA) discriminated carrier to construct CRISPR/Cas signal reporter system. The combination of recombinase polymerase amplification with MoS2 NSs modulated CRISPR/Cas12a helped achieve attomolar sensitivity for nucleic acid detection within 35 min. Moreover, the results were obtained using a portable apparatus, enabling visual detection at the point of care. The practical applicability of this biosensing platform was successfully achieved through the detection of anisakis in real samples. This study provides novel insights into exploring the feasibility of two-dimensional nanomaterials based reporter in the CRISPR/Cas12a system, as well as offers a reliable tool for on-site monitoring of parasites.
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