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Rapid detection of H146-like goose calicivirus using a TaqMan-based real-time PCR assay

文献类型： 外文期刊

作者： Zheng, Min 1 ; Lin, Su 1 ; Zhang, Shizhong 1 ; Chen, Xiuqin 1 ; Jiang, Dandan 1 ; Chen, Shaoying 1 ; Wang, Shao 1 ; Chen, 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou, Peoples R China

2.Fujian Acad Agr Sci, Fujian Anim Dis Control Technol Dev Ctr, Fuzhou, Peoples R China

关键词： goose calicivirus; H146-like; real-time PCR; TaqMan probe; virus detection

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

ISSN：

年卷期： 2021 年 100 卷 2 期

页码：

收录情况： SCI

摘要： H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was recently discovered and proposed to cause runting-stunting syndrome and urate deposition in geese. At present, however, there is a lack of epidemiological information pertaining to the dynamics and distribution of H146-like GCV. The development of novel molecular diagnostic approaches capable of rapidly and accurately detecting this virus would support the strengthening, the prevention, and control of H146-like GCV infection. In the present study, we therefore used a TaqMan probe and primers specific for the viral nonstructural (NS) gene to develop a highly sensitive and specific PCR assay capable of detecting this H146-like GCV. The assay reproducibly detected 5.07 x 10(2) copies of a recombinant DNA plasmid containing the NS gene, with a dynamic range of 8 orders of magnitude (10(2)-10(9) copies). Importantly, no cross-reactivity was observed with common viruses that affected waterfowl, and when we used this assay to evaluate clinical samples, we found it to be more sensitive and faster than traditional PCR. In summary, herein, we developed a novel TaqMan-based real-time PCR approach that could reliably detect and diagnose H146-like GCV. This tool will allow for the real-time diagnosis of H146-like GCV infections, enabling researchers to better understand the epidemiology and clinical presentation of this disease.

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