文献类型： 外文期刊
作者： Zhang, He 1 ; Yang, Zhichang 1 ; Shao, Jianzhu 1 ; Sun, Jianshe 1 ; Zha, Qian 2 ; Zhang, Xueying 1 ;
作者机构： 1.Hebei Agr Univ, Coll Hort, Baoding 071001, Peoples R China
2.Shanghai Acad Agr Sci, Res Inst Forestry & Pomol, Shanghai 201403, Peoples R China
关键词： apple; dwarf; RNA-seq; MdWRKY24; MdRGL; Malus domestica; Fuji; Baleng Crab; Arabidopsis thaliana
期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）
ISSN：
年卷期： 2022 年 12 卷 10 期
页码：
收录情况： SCI
摘要： In apple cultivation, dwarf rootstocks are chosen for dense planting and intensive cultivation, which is beneficial to production management. Dwarf rootstocks are widely used in apple production in China. However, the dwarfing mechanisms of dwarf interstock are still unclear. Here, M9 and SH40 were selected as the dwarf interstocks for potted Fuji apples. The key transcription factor MdWRKY24 was screened via transcriptional sequencing. The open reading frame sequence of the MdWRKY24 gene was 657 bp in length, encoded 218 amino acids, and was located on the cell membrane. The MdWRKY24-overexpressing Arabidopsis line showed a dwarf phenotype and delayed flowering. The DELLA protein RGA-like (RGL) gene is a repressor of the gibberellin signaling pathway. Yeast two-hybrid analysis revealed that MdWRKY24 could interact with MdRGL1/2/3. The results indicated that MdWRKY24 might affect plant dwarfing through the synergistic effect of MdRGL1/2/3. The MdWRKY24-MdRGL may be an important pathway underlying the gibberellin-mediated regulation of apple dwarfing.
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