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Preparation of Pseudo-typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

文献类型： 外文期刊

作者： Chang, Xiaoyan 1 ; Zhou, Keru 1 ; Liu, Yanguang 1 ; Duan, Lian 3 ; Zhang, Lichun 2 ; Zhang, Guoliang 3 ; Wang, Haikun 4 ; Wang, Guiqin 1 ;

作者机构： 1.Nanjing Adv Acad Life & Hlth, Nanjing, Peoples R China

2.Jilin Acad Agr Sci, Inst Anim Biotechnol, Changchun, Peoples R China

3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China

4.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Pasteur Shanghai, CAS Key Lab Mol Virol & Immunol, Beijing, Peoples R China

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.424； 五年影响因子：1.789 ）

ISSN： 1940-087X

年卷期： 2021 年 177 期

页码：

收录情况： SCI

摘要： Since 1996, A/goose/Guangdong/1/96-lineage highly pathogenic avian influenza (HPAI) H5 viruses have been causing flu outbreaks in poultry and wild birds. Occasionally, humans also fall victim to it, which results in high mortality. Nonetheless, HPAI virus research is often hindered, considering that it must be handled within biosafety level 3 laboratories. To address this issue, pseudoviruses are adopted as an alternative to wild-type viruses in some experiments of H5 HPAI studies. Pseudoviruses prove to be the ideal tools to study neutralizing antibodies against H5 HPAI viruses. This protocol describes the procedures and critical steps of H5 HPAI pseudovirus preparations and pseudovirus neutralization assays. Also, it discusses the troubleshooting, limitation, and modifications of these assays.

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