文献类型： 外文期刊

作者： Chen, Mengdi ¹ ; Zhang, Congcong ³ ; Wu, Zewen ¹ ; Guo, Siwei ¹ ; Lv, Wenfa ⁴ ; Song, Jixuan ¹ ; Hao, Beibei ¹ ; Bai, Jinhui ¹ ; Zhang, Xinxin ¹ ; Xu, Hongyan ¹ ; Xia, Guangjun ¹ ;

作者机构： 1.Yanbian Univ, Coll Agr, Yanji 133000, Peoples R China

2.Yanbian Univ, Coll Integrat Sci, Yanji 133000, Peoples R China

3.Jilin Acad Agr Sci, Inst Anim Sci, Changchun 136100, Peoples R China

4.Jilin Agr Univ, Minist Educ, Joint Lab Modern Agr Technol Int Cooperat, Changchun 130118, Peoples R China

5.Yanbian Univ, Res Ctr North East Cold Reg Beef Cattle Sci & Tech, Yanji 133000, Jilin, Peoples R China

关键词： AMPK/mTOR Signalling Pathway; bta-miR-365-3p; FK506-binding protein 5 FKBP5 ); Preadipocytes; Yanbian Yellow Cattle

期刊名称：ANIMAL BIOSCIENCE （ 影响因子：2.4； 五年影响因子：2.4 ）

ISSN： 2765-0189

年卷期： 2024 年 37 卷 7 期

页码：

收录情况： SCI

摘要： Objective: MicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood. Methods: We identified bta-miR-365-3p, which specifically targets the 3 ' untranslated region (3 ' UTR) of the FK506-binding protein 5 (FKBP5), and verified its mechanisms for regulating expression and involvement in adipogenesis. Results: In this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-36 5-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBP alpha and PPAR gamma. The dualluciferase reporter system further validated the targeting relationship between bta-miR365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by quantitative real-time polymerase chain reaction and Western blot. Over expression of bta-miR-3653p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. Adenosine 5 '-monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/ mTOR) signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-3653p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results. Conclusion: In conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.