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Sequence, structure, and function of the Dps DNA-binding protein from Deinococcus wulumuqiensis R12

文献类型： 外文期刊

作者： Chen, Yao 1 ; Yang, Zhihan 2 ; Zhou, Xue 1 ; Jin, Mengmeng 1 ; Dai, Zijie 1 ; Ming, Dengming 2 ; Zhang, Zhidong 2 ; Zhu, Liying 3 ; Jiang, Ling 1 ;

作者机构： 1.Nanjing Tech Univ, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China

2.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Peoples R China

3.Nanjing Tech Univ, Sch Chem & Mol Engn, Nanjing 211816, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Key Lab Special Environm Microbiol, Urumqi 830091, Xinjiang, Peoples R China

关键词： Deinococcus wulumuqiensis R12; DNA-binding protein; Oxidative stress; Proteomics

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：6.352； 五年影响因子：6.496 ）

ISSN：

年卷期： 2022 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Deinococcus wulumuqiensis R12, which was isolated from arid irradiated soil in Xinjiang province of China, belongs to a genus that is well-known for its extreme resistance to ionizing radiation and oxidative stress. The DNA-binding protein Dps has been studied for its great contribution to oxidative resistance. To explore the role of Dps in D. wulumuqiensis R12, the Dps sequence and homology-modeled structure were analyzed. In addition, the dps gene was knocked out and proteomics was used to verify the functions of Dps in D. wulumuqiensis R12. Docking data and DNA binding experiments in vitro showed that the R12 Dps protein has a better DNA binding ability than the Dps1 protein from D. radiodurans R1. When the dps gene was deleted in D. wulumuqiensis R12, its resistance to H2O2 and UV rays was greatly reduced, and the cell envelope was destroyed by H2O2 treatment. Additionally, the qRT-PCR and proteomics data suggested that when the dps gene was deleted, the catalase gene was significantly down-regulated. The proteomics data indicated that the metabolism, transport and oxidation-reduction processes of D. wulumuqiensis R12 were down-regulated after the deletion of the dps gene. Overall, the data conformed that Dps protein plays an important role in D. wulumuqiensis R12.

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