文献类型： 外文期刊
作者： Wang, Zhijun 1 ; Cai, Danfeng 1 ; Li, Kan 1 ; Ju, Xing 1 ; Nie, Qinghua 1 ;
作者机构： 1.South China Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci, Guangzhou 510642, Peoples R China
2.Minist Agr, Natl Local Joint Engn Res Ctr Livestock Breeding, Guangdong Prov Key Lab Agro Animal Genom & Mol B, Guangzhou 510642, Peoples R China
3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Peoples R China
关键词： cycloleucine; N6-Methyladenosine; myogenesis; mRNA-Seq; chicken
期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0032-5791
年卷期： 2022 年 101 卷 12 期
页码：
收录情况： SCI
摘要： N6-Methyladenosine (m(6)A) has been reported to involve and play an important role in various biological activities but seldom in poultry myogenesis. Cycloleucine usually functions as a nucleic acid methylation inhibitor, the inhibition efficiency of cycloleucine at the m(6)A level and corresponding dynamic changes of poultry muscle cells remain unknown. In this study, we aim to find out the effect of cycloleucine on the total N6-Methyladenosine level and its molecular mechanism for regulating myogenesis. A total of 745 differentially expressed genes (DEGs) were obtained by 10 mM, 20 mM, and 30 mM of cycloleucine treatment compared with 0 mM treatment. DEGs in 10 mM cycloleucine were significantly enriched in the biological process of skeletal muscle and satellite cell proliferation and differentiation, DEGs in 20 and 30 mM cycloleucine were enriched in some metabolic and biosynthetic processes. The trend analysis showed that 85% of all DEGs were significantly clustered into 4 files, among them 59% DEGs were dose-dependent and 26% were dose-independent, 52% DEGs were in down-trend and 33% DEGs were in uptrend. Also, the cycloleucine treatment could trigger cell cycle arrest in the G1 phase and depress myoblast cell proliferation and inhibit myotube formation. In conclusion, cycloleucine could continuously reduce the m(6)A level of myoblast cells, depress myoblast cell proliferation and inhibit myotube formation.
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