文献类型： 外文期刊
作者： Xu, Xia 1 ; Wang, Yaohui 2 ; Chen, Jine 1 ; Du, Xin 1 ; Yao, Lusong 1 ; Xu, Jun 1 ; Zhang, Yong 2 ; Huang, Yongping 2 ; Wang, Yongqiang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Sericulture & Tea, Hangzhou, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Insect Dev & Evolutionary Biol, Shanghai, Peoples R China
关键词： BmSer1; DNA molecule mutation; male sterility; Bombyx mori; CRISPR; Cas9
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.755； 五年影响因子：5.316 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Serine proteases are important in reproduction, embryonic development, cell differentiation, apoptosis, and immunity. The genes encoding some serine proteases are essential for male fertility in both humans and rodents and are functionally conserved among metazoan. For example, the Serine protease 1 (Ser1) gene determines male reproductive success in the model lepidopteran insect Bombyx mori. In this study, we explored the function of BmSer1 through transgenic CRISPR/Cas9 technology-mediated mutations in silkworm. We found that the mutation of BmSer1 gene resulted in male sterility but had no effect on female fertility. Male mutants produce normal eupyrene sperm bundles, but the sperm bundles do not dissociate into single sperm. Male sterility caused by the BmSer1 gene mutation was inherited stably through female individuals. Therefore, the serine protease encoded by BmSer1 is essential for male reproductive success in lepidopterans and is a potential target gene for biological reproductive regulation.
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