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Profiles of cyclin B and cdc2 during ovarian and embryonic development in Exopalaemon carinicauda

文献类型： 外文期刊

作者： Liang, Junping 1 ; Sun, Xiaotong 1 ; Li, Shuangfeng 1 ; Li, Yanling 1 ; Li, Hui 1 ; Wang, Xianfeng 1 ; Li, Jian 2 ; Li, Jitao 2 ;

作者机构： 1.Henan Normal Univ, Coll Fisheries, Engn Technol Res Ctr Henan Prov Aquat Anim Cultiva, Xinxiang 453007, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： cyclin B; cdc2; embryonic development; Exopalaemon carinicauda; ovarian development

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 2022影响因子：0.6； 五年影响因子：0.6 ）

ISSN： 0792-156X

年卷期： 2023 年 75 卷

页码：

收录情况： SCI

摘要： Mitosis-promoting factor (MPF) is a complex formed by cyclin B (cyclin B) and cyclin-dependent kinase (cdc2). To investigate the role of MPF in the reproduction of Exopalaemon carinicauda, we cloned its full-length cDNA of the Ec-cyclin B and Ec-cdc2 genes. We analyzed their molecular characteristics and expression profiles during ovarian and embryonic development. The results showed that the Ec-cyclin B gene was 1194 bp long and encoded a 397 amino acid (aa) long protein. However, Ec-cdc2 was 900 bp long, which encoded 299 aa with a conserved cyclin binding motif PSTAIRE. The phylogenetic tree analysis showed that Ec-cyclin B had the highest homology with the cyclin B of Macrobrachium rosenbergii (81.06%). In comparison, Ec-cdc2 had the highest homology with the cdc2 of E. modestus (96.80%). Ec-cyclin B showed the highest expression in the ovary, whereas Ec-cdc2 was the highest in the hepatopancreas, followed by the ovary. In the five stages of ovarian development, Ec-cyclin B and Ec-cdc2 expression levels reach the highest at stage V(p < 0.05). Overall, the expression of these two genes first increased and then decreased at different embryonic developmental stages. Therefore, these findings suggested that cyclin B and cdc2 played an essential role in the ovarian and embryonic development of E. carinicauda.

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