您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of Recombinant MAP30 on Cell Proliferation and Apoptosis of Human Colorectal Carcinoma LoVo Cells

文献类型： 外文期刊

作者： Fan, Jian-Ming 2 ; Luo, Jun 1 ; Xu, Jun 3 ; Zhu, Sha 4 ; Zhang, Qiao 2 ; Gao, De-Fu 5 ; Xu, Yu-Bao 2 ; Zhang, Gai-Ping 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Zhengzhou Univ, Coll Publ Hlth, Toxicol Lab, Zhengzhou, Peoples R China

3.Henan Agr Univ, Sch Life Sci, Zhengzhou 450002, Peoples R China

4.Zhengzhou Univ, Coll Basic Med Sci, Dept Microbiol & Immunol, Zhengzhou, Peoples R China

5.Henan Univ Tradit Chinese Med, Coll Basic Med Sci, Zhengzhou 450008, Peoples R China

关键词： Apoptotic;Bitter melon;Comet assay;LoVo cells;MAP30

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MAP30, an attractive protein isolated from bitter melon, has been previously found to have the anti-tumor and anti-HIV activities. In this study, MAP30 was cloned and expressed and the effects of the recombinant protein on cell proliferation and apoptosis of human colorectal carcinoma LoVo cells were investigated. The results showed that the proliferation of LoVo cells were significantly suppressed by MAP30 in time- and dose-dependent manners at the concentration ranging from 0.67 to 4.67 μM. The apoptotic nuclei of LoVo cells induced by MAP30 were obviously observed, and the genomic degradation was detected by single-cell gel electrophoresis (comet assay). Nuclear condensation and boundary aggregation or split, apoptotic bodies were seen by fluorescence and electron microscopy. The proportion of the periodic tumor cells was altered by MAP30. Sub-G1 curves were displayed by a flow cytometry analysis. Results of northern and western blots showed that the transcription and expression of Bax, a member of pro-apoptotic proteins, were gradually up-regulated as treated time increased. On the contrary, the transcription and expression of Bcl-2, an anti-apoptotic member, were down-regulated. These data provided powerful evidences for the first time that recombinant MAP30 can induce the apoptosis of the human colorectal carcinoma LoVo cells.

  • 相关文献

[1]苫瓜蛋白MAP30在毕赤酵母中的表达及其诱发胃腺癌细胞MCG803凋亡的研究. 樊剑鸣,朱沙,罗俊,张改平. 2009

[2]苦瓜蛋白MAP30在毕赤酵母中的表达及其诱发胃腺癌细胞MCG803凋亡的研究. 樊剑鸣,朱沙,罗俊,张改平. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台