Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions
文献类型： 外文期刊
作者： Yang, Yibin 1 ; Zhu, Xia 1 ; Zhang, Haixin 3 ; Chen, Yuhua 4 ; Song, Yi 2 ; Ai, Xiaohui 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
2.Minist Agr, Key Lab Qual & Safety Control Aqut Prod, Beijing, Peoples R China
3.Jiangxi Fisheries Res Inst, Fish Dis Lab, Nanchang, Jiangxi, Peoples R China
4.Wuhan Univ, Dept Gastroenterol, Zhongnan Hosp, Wuhan, Peoples R China
5.Wuhan Univ, Hubei Clin Ctr, Zhongnan Hosp, Wuhan, Peoples R China
6.Wuhan Univ, Key Lab Intestinal & Colorectal Dis, Zhongnan Hosp, Wuhan, Peoples R China
关键词： channel catfish; Yersinia ruckeri; dual RNA-seq; host-pathogen interactions; immunity; virulence
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：8.786； 五年影响因子：8.876 ）
ISSN： 1664-3224
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with Yersinia ruckeri. The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. Y. ruckeri mainly promote pathogenesis through the flagellum gene fliC in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish.
- 相关文献
作者其他论文 更多>>
-
Molecular mechanism of Yersinia ruckeri Flagellin C (FliC) induced intestinal inflammation in channel catfish ( Ictalurus punctatus)
作者：Yang, Yibin;He, Hao;Ai, Xiaohui;Zhu, Xia;Huang, Yucong;Yin, Fei;Xu, Jingen;Chen, Yuhua
关键词：Channel catfish; Yersinia ruckeri; Virulence; Intestinal inflammation; Molecular mechanism
-
Characteristics and functions of interleukin 21 in channel catfish (Ictalurus punctatus)
作者：He, Hao;He, Hao;Zhu, Xia;Dong, Jing;Yang, Qiuhong;Ai, Xiaohui;Yang, Yibin;Deng, Ping;Huang, Yucong;Wang, Hui
关键词：Channel catfish; IL-21; Immune response; Inflammatory factors; Gene expression
-
Population Pharmacokinetics of Enrofloxacin in Ctenopharyngodon idella Based on the Sparse Sampling Method and a Nonlinear Mixed Effect Model Following Intravenous and Oral Administration
作者：Xu, Ning;Zhang, Huan;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Xu, Ning;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Dong, Jing;Ai, Xiaohui;Zhang, Huan;Ding, Yongzhen
关键词：bioavailability; enrofloxacin; grass carp; population pharmacokinetics
-
Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass
作者：Yang, Yibin;He, Hao;Yang, Qiuhong;Ai, Xiaohui;Zhu, Xia;Deng, Ping
关键词：Streptococcus iniae; Largemouth bass; Whole genome; Virulence; Drug resistance
-
Population Pharmacokinetics of Enrofloxacin in Micropterus salmoides Based on a Nonlinear Mixed Effect Model After Intravenous and Oral Administration
作者：Xu, Ning;Zhou, Shun;Dong, Jing;Li, Jiangtao;Ai, Xiaohui;Xu, Ning;Ding, Yongzhen
关键词：enrofloxacin; population pharmacokinetics; statistical approaches; largemouth bass; body weight
-
Preclinical Evaluation of Fenbendazole for Controlling Gyrodactylus kobayashii (Monogenea, Gyrodactylidae) in Goldfish: Dose Optimization and Safety Assessment
作者：Dong, Jing;Li, Jiangtao;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Ai, Xiaohui;Zhou, Shun;Dong, Jing
关键词：preclinical study; fenbendazole; monogenean; anthelmintic treatment; aquaculture
-
Novel Insights into the Therapeutic Effect of Amentoflavone Against Aeromonas hydrophila Infection by Blocking the Activity of Aerolysin
作者：Dong, Jing;Li, Shengping;Zhou, Shun;Liu, Yongtao;Yang, Qiuhong;Xu, Ning;Yang, Yibin;Ai, Xiaohui;Li, Shengping;Cheng, Bo
关键词：
Aeromonas hydrophila ; aerolysin; amentoflavone; molecular dynamics; anti-infective
