文献类型： 外文期刊

作者： Zhang, Bi-zhou ¹ ; Borjigin, Qinggeer ¹ ; Gao, Ju-lin ¹ ; Yu, Xiao-fang ¹ ; Hu, Shu-ping ¹ ; Wang, Fu-gui ¹ ; Zhang, Xin ¹ ; Han, Sheng-cai ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Agr Coll, Hohhot, Peoples R China

2.Inner Mongolia Key Lab Crop Cultivat & Genet Impro, Hohhot, Peoples R China

3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China

关键词： metagenomic; microbial consortium; low temperature; corn stover degradation

期刊名称：JOURNAL OF ENVIRONMENTAL ENGINEERING AND LANDSCAPE MANAGEMENT （ 影响因子：1.3； 五年影响因子：2.2 ）

ISSN： 1648-6897

年卷期： 2023 年 31 卷 1 期

页码：

收录情况： SCI

摘要： In our previous work, a microbial consortium GF-20 (Qinggeer et al., 2016) was enriched from compost habi-tats and adapted to efficiently and stably degrade corn stover under low temperatures. While the main microorganism and degradation-related functions and degradation-related coding enzyme genes of GF-20 were not clear. Therefore, the cur-rent study used the metagenomic to decipher the systematic and functional contexts within such microbial consortium un-der low temperatures. The results showed that the dominant functional microbials in GF-20 consortium were bacteria. The dominant phylums in GF-20 consortium were Proteobacteria (62.84%) and Bacteroidetes (10.24%). The dominant genus was Pseudomonas (50.84%), followed by Dysgonomonas (5.86%), Achromobacter (4.94%), Stenotrophomonas (3.67%) and Flavo-bacterium (2.04%). The metabolism was mainly composed of carbohydrate metabolism and amino acid metabolism, and in-cluded signal transduction, cell transport and other metabolic modes. The functional genes encoded were mainly distributed in glycosidolytic enzyme genes, and the functional enzymes were beta-glucosidase, acetyl-CoA, pyruvate dehydrogenase and galactosidase. The GF-20 microbial consortium degraded the cellulose in corn stover primarily by beta-glucosidase and endoglu-canase, which were produced by 12 genera of microorganisms. The hemicellulose synergistic effect was produced by 15 genera of microorganisms including xylanase, xyloglucanase, mannolanase and branching enzyme.