文献类型： 外文期刊

作者： Zhang, Xin ¹ ; Borjigin, Qinggeer ¹ ; Gao, Ju-Lin ¹ ; Yu, Xiao-Fang ¹ ; Hu, Shu-Ping ³ ; Zhang, Bi-Zhou ⁶ ; Han, Sheng-Cai ³ ;

作者机构： 1.Inner Mongolia Agr Univ, Agr Coll, 275 XinJian East St, Hohhot 010019, Peoples R China

2.TongHua Normal Univ, Life Sci Coll, 950 YuCai Rd, Tonghua 314002, Peoples R China

3.Key Lab Crop Cultivat & Genet Improvement Inner M, 275 XinJian East St, Hohhot 010019, Peoples R China

4.Inner Mongolia Agr Univ, Hort & Plant Protect Coll, 29 Eerduosi East St, Hohhot 010019, Peoples R China

5.Inner Mongolia Agr Univ, Vocat & Tech Coll, Altan St, Baotou 014109, Peoples R China

6.Inner Mongolia Acad Agr Anim Husb Sci, Special Crops Inst, 22 ZhaoJun Rd, Hohhot 010031, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.996； 五年影响因子：5.516 ）

ISSN： 2045-2322

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： To systematically explore and analyze the microbial composition and function of microbial consortium M44 with straw degradation in the process of subculture at low temperature. In this study, straw degradation characteristics of samples in different culture stages were determined. MiSeq high-throughput sequencing technology was used to analyze the evolution of community structure and its relationship with degradation characteristics of microbial consortium in different culture periods, and the PICRUSt function prediction analysis was performed. The results showed that straw degradation rate, endoglucanase activity, and filter paper enzyme activity of M44 generally decreased with increasing culture algebra. The activities of xylanase, laccase, and lignin peroxidase, as well as VFA content, showing a single-peak curve change with first an increase and then decrease. In the process of subculture, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in different culture stages. Pseudomonas, Flavobacterium, Devosia, Brevundimonas, Trichococcus, Acinetobacter, Dysgonomonas, and Rhizobium were functional bacteria in different culture stages. It was found by PICRUSt function prediction that the functions were concentrated in amino acid transport and metabolism, carbohydrate transship and metabolism related genes, which may contain a large number of fibers and lignin degrading enzyme genes. In this study, the microbial community succession and the gene function in different culture periods were clarified and provide a theoretical basis for screening and rational utilization of microbial consortia.