The P3N-PIPO Protein Encoded by Wheat Yellow Mosaic Virus Is a Pathogenicity Determinant and Promotes Its Pathogenicity through Interaction with NbRLK6 in Nicotiana benthamiana
文献类型： 外文期刊
作者： Miao, Runpu 1 ; Ye, Zhuangxin 1 ; MacFarlane, Stuart 2 ; Li, Yanjun 1 ; Mao, Qianzhuo 1 ; Tian, Yanzhen 1 ; Deng, Zhiping 3 ; Sun, Zongtao 1 ; Yang, Jian 1 ; Li, Junmin 1 ; Yan, Fei 1 ; Chen, Jianping 1 ; Yu, Chulang 1 ;
作者机构： 1.Ningbo Univ, Key Lab Biotechnol Plant Protect, State Key Lab Managing Biot & Chem Threats Qual &, Inst Plant Virol,Minist Agr & Zhejiang Prov, Ningbo 315211, Peoples R China
2.James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, Scotland
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
关键词： WYMV; P3N-PIPO; pathogenicity; NbRLK6
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Similarly to other potyvirids, the bymovirus wheat yellow mosaic virus (WYMV) encodes a P3N-PIPO protein that is expressed by frameshifting occurring within the open reading frame of the P3 protein. P3N-PIPO is known to be essential for the cell-to-cell movement of several potyviruses, but this has not yet been confirmed for the WYMV. Here, we show that the WYMV P3N-PIPO protein influences disease symptom formation. Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the WYMV P3N-PIPO gene induced more severe disease symptoms and resulted in higher virus accumulation levels than did infection with PVX lacking the P3N-PIPO gene. N. benthamiana P3N-PIPO-interacting proteins were identified through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS (mass spectrometry), and the interaction between P3N-PIPO and the N. benthamiana receptor-like kinase NbRLK6 was further verified by Co-IP and bimolecular fluorescence complementation (BiFC) of transiently-expressed proteins. Furthermore, our investigation showed that the disease symptom severity and accumulation level of PVX-P3N-PIPO were decreased in N. benthamiana plants when NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencing.
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