Development of an Online Tool for Pasteurella multocida Genotyping and Genotypes of Pasteurella multocida From Different Hosts
文献类型： 外文期刊
作者： Peng, Zhong 1 ; Liu, Junyang 2 ; Liang, Wan 1 ; Wang, Fei 1 ; Wang, Li 2 ; Wang, Xueying 1 ; Hua, Lin 1 ; Chen, Huanchun 1 ; Wilson, Brenda A. 4 ; Wang, Jia 2 ; Wu, Bin 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Cooperat Innovat Ctr Sustainable Pig Prod, State Key Lab Agr Microbiol, Wuhan, Peoples R China
2.Huazhong Agr Univ, Coll Informat, Hubei Key Lab Agr Bioinformat, Wuhan, Peoples R China
3.Hubei Acad Agr Sci, Anim Husb & Vet Inst, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr & Rural Affairs, Wuhan, Peoples R China
4.Univ Illinois, Sch Mol & Cellular Biol, Dept Microbiol, Urbana, IL USA
关键词： Pasteurella multocida; genotyping; whole genome sequence; PmGT; genotypes
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）
ISSN：
年卷期： 2021 年 8 卷
页码：
收录情况： SCI
摘要： Pasteurella multocida is a versatile zoonotic pathogen. Multiple systems have been applied to type P. multocida from different diseases in different hosts. Recently, we found that assigning P. multocida strains by combining their capsular, lipopolysaccharide, and MLST genotypes (marked as capsular: lipopolysaccharide: MLST genotype) could help address the biological characteristics of P. multocida circulation in different hosts. However, there is still lack of a rapid and efficient tool to diagnose P. multocida according to this system. Here, we developed an intelligent genotyping platform PmGT for P. multocida strains according to their whole genome sequences using the web 2.0 technologies. By using PmGT, we determined capsular genotypes, LPS genotypes, and MLST genotypes as well as the main virulence factor genes (VFGs) of P. multocida isolates from different host species based on their whole genome sequences published on NCBI. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. With the advent of high-quality, inexpensive DNA sequencing, PmGT represents a more efficient tool for P. multocida diagnosis in both epidemiological studies and clinical settings.
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