您好,欢迎访问中国水产科学研究院 机构知识库!

Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate

文献类型: 外文期刊

作者: Du, Meng 1 ; Chen, Jixiang 2 ; Zhang, Xiaohua 2 ; Li, Aijuan 2 ; Li, Yun 2 ; Wang, Yingeng 2 ;

作者机构: 1.Ocean Univ China, Coll Marine Life Sci, Dept Marine Biol, Qingdao 266003, Peoples R China

2.Ocean Univ China, Coll Marine Life Sci, Dept Marine Biol, Qingdao 266003, Peoples R China; Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词: NON-CULTURABLE VIBRIO;SHIGELLA-DYSENTERIAE TYPE-1;STRESSED ESCHERICHIA-COLI;CAMPYLOBACTER-JEJUNI;IN-VIVO;STATE;RESUSCITATION;ENVIRONMENT;SURVIVAL;VULNIFICUS

期刊名称:APPLIED AND ENVIRONMENTAL MICROBIOLOGY ( 影响因子:4.792; 五年影响因子:5.26 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4 degrees C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (< 0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 10(9) CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity.

  • 相关文献

[1]Nonisothermal crystallization kinetics of poly(ethylene terephthalate)/antimony-doped tin oxide nanocomposites. Chen, Xiaolei,Li, Chunzhong,Shao, Wei,Chen, Xiaolei,Wang, Lumin,Liu, Tianxi.

[2]Investigation of the interaction between the fate of antibiotics in aquafarms and their level in the environment. Zhong, Yuanhong,Chen, Zhi-Feng,Liu, Shuang-Shuang,Liu, Guoguang,Cai, Zongwei,Chen, Zhi-Feng,Dai, Xiaoxin,Zheng, Guangming,Zhu, Xinping,Liu, Shugui,Yin, Yi,Cai, Zongwei. 2018

[3]TOXICITY TESTING OF CRUDE OIL AND FUEL OIL USING EARLY LIFE STAGES OF THE BLACK PORGY (ACANTHOPAGRUS SCHLEGELII). Shen, Anglv,Tang, Fenghua,Shen, Xinqiang,Xu, Wantu.

[4]Levels, tissue distribution, and age-related accumulation of synthetic musk fragrances in Chinese sturgeon (Acipenser sinensis): Comparison to organochlorines. Wan, Yi,Wei, Qiwei,Hu, Jianying,Jin, Xiaohui,Zhang, Zhaobin,Zhen, Huajun,Liu, Jianyi.

[5]1-Deoxynojirimycin from Bacillus subtilis improves antioxidant and antibacterial activities of juvenile Yoshitomi tilapia. Tang, Lining,Huang, Kai,Sun, Lei,Huang, Qing,Bi, Yanjun,Xie, Jun,Yu, Dan. 2017

[6]The effect of temperature and salinity on survival and growth of Crangon uritai (Decapoda : Crangonidae) larvae reared in the laboratory. Li, Hui Yu,Hong, Sung Yun. 2007

[7]Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei. Niu, Jin,Lin, Hei-Zhao,Chen, Peng-Fei,Tian, Li-Xia,Liu, Yong-Jian,Yang, Hui-Jun,Liang, Gui-Ying. 2012

[8]Effects of bioflocs on water quality, and survival, growth and digestive enzyme activities of Litopenaeus vannamei (Boone) in zero-water exchange culture tanks. Xu, Wu Jie,Pan, Lu Qing,Sun, Xiao Hua,Huang, Jie. 2013

[9]Effects of algae and live food density on the feeding ability, growth and survival of miiuy croaker during early development. Shan, Xiujuan,Lin, Meijin,Shan, Xiujuan. 2014

[10]Effects of dietary chitosan on growth, survival and stress tolerance of postlarval shrimp, Litopenaeus vannamei. Niu, J.,Liu, Y-J,Yang, H-J,Liang, G-Y,Tian, L-X,Niu, J.,Lin, H-Z,Mai, K-S. 2011

[11]Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus. Yin, Fei,Sun, Peng,Gao, Quanxin,Shi, Zhaohong,Peng, Shiming,Gong, Qiyang,Li, Yanwei,Li, Anxing,Dan, Xueming. 2014

[12]Criteria for assessing juvenile quality of the lined seahorse, Hippocampus erectus. Zhang, Dong,Yin, Fei,Zhang, Dong,Lin, Junda. 2011

[13]Food ingestion, prey selectivity, feeding incidence, and performance of yellowtail kingfish Seriola lalandi larvae under constant and varying temperatures. Ma, Zhenhua,Ma, Zhenhua. 2014

[14]Larval development and salinity tolerance of Japanese flounder (Paralichthys olivaceus) from hatching to juvenile settlement. Wang, Youji,Guo, Qindan,Zhao, Hu,Lu, Weiqun,Liu, Haijin.

[15]Suitable dietary chitosan improves the growth performance, survival and immune function of tiger shrimp, Penaeus monodon. Niu, Jin,Li, Chun-Hou,Chen, Xu,Wu, Kai-Chang,Jun, Wang,Huang, Zhong,Wang, Yun,Lin, Hei-Zhao,Tian, Li-Xia,Liu, Yong-Jian.

[16]Combined effects of carbonate alkalinity and pH on survival, growth and haemocyte parameters of the Venus clam Cyclina sinensis. Lin, Tingting,Lai, Qifang,Yao, Zongli,Lu, Jianxue,Zhou, Kai,Wang, Hui.

[17]Genetic parameters and genotype by environment interaction for cold tolerance, body weight and survival of the Pacific white shrimp Penaeus vannamei at different temperatures. Li, Wenjia,Luan, Sheng,Luo, Kun,Sui, Juan,Xu, Xiaodong,Tan, Jian,Kong, Jie.

[18]Heritability and genetic correlation of survival in turbot (Scophthalmus maximus). Wang Xin'an,Ma Aijun,Huang Zhihui,Zhou Zhou. 2010

[19]Effect of inbreeding on survival, WSSV tolerance and growth at the postlarval stage of experimental full-sibling inbred populations of the Chinese shrimp Fenneropenaeus chinensis. Luo, Kun,Luo, Kun,Kong, Jie,Luan, Sheng,Meng, Xian-Hong,Zhang, Tian-Shi,Wang, Qing-Yin. 2014

[20]Dietary supplementation of honeysuckle improves the growth, survival and immunity of Penaeus monodon. Chen, Xu,Lin, Hei-Zhao,Jiang, Shi-Gui,Wu, Kai-Chang,Niu, Jin,Liu, Yong-Jian,Tian, Li-Xia,Zhang, Yun-Qiang. 2013

作者其他论文 更多>>