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Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation

文献类型： 外文期刊

作者： Li, Dequan 1 ; Nie, Fengya 2 ; Wei, Lihui 2 ; Wei, Benqiang 2 ; Chen, Zhiyi 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China; Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Peoples R China

关键词： screening;high-yielding mutant;ion implantation;SOILBORNE PLANT-PATHOGENS;BACILLUS-SUBTILIS;RHIZOCTONIA-SOLANI;BIOLOGICAL-CONTROL;SEED TREATMENT;DAMPING-OFF;BEAM;ACID;ENHANCEMENT;RHIZOSPHERE

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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收录情况： SCI

摘要： Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N+ implantation. The optimum dose of ion implantation for the Bs-916 was from 15 x 2.6 x 10(14) N+ cm(2) to 25 x 2.6 x 10(14) N+/cm(2). The mutant strain designated as Bs-H74 was obtained, which showed higher inhibition activity in the screening plate. Its inhibition zone against the indicator organism increased by 30.7% compared to the parental strain. The control effect of rice sheath blight was improved by 14.6% over that of Bs-916. Thin-layer chromatography and high-performance liquid chromatography analysis indicated that lipopeptides produced by Bs-916 and the mutant strains belonged to the surfactin family. Bs-H74 produced approximately 3.0-fold surfactin compared to that of Bs-916. To determine the role of surfactin in biocontrol by Bs-916, we tested another mutant strain, Bs-M49, which produced lower levels of surfactin significantly, and found that Bs-M49 had no obvious effects against R. solani. These results suggested that the surfactin produced by Bs-916 plays an important role in the suppression of sheath blight. These observations also showed that the Bs-H74 mutant strain is a better biocontrol agent than the parental strain.

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