您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues

文献类型： 外文期刊

作者： Zhao, Yinli 1 ; Zhang, Gaiping 1 ; Liu, Qingtang 1 ; Teng, Man 1 ; Yang, Jifei 1 ; Wang, Jianhua 3 ;

作者机构： 1.Henan Acad Agr Sci, Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China

3.NW A&F Univ, Coll Vet Med, Yangling 712100, Shanxi, Peoples R China

关键词： Enrofloxacin;test strip;colloidal gold;immunoassay;rapid test

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A rapid immunochromatographic lateral flow test strip of competitive format has been developed using a gold-conjugated monoclonal antibody for the specific determination of enrofloxacin (ENR) residues in chicken muscles. For this purpose, a specific monoclonal antibody (mAb) for ENR was generated and characterized. The mAb showed negligible cross-reactivity with other related compounds. Using ENR standards prepared in chicken muscle extracts from 0 to 24.3 ng/mL (μg/ kg), the method indicated that the detection limit of the test strip, as measured in a strip scanner, was as low as 0.138 μg/kg of ENR and the half-maximal inhibition concentration (IC_(50)) was 0.935 μg/kg. For samples spiked at 10, 20, and 30 μg/kg, the recovery was between 85.3 and 96.1% and the coefficient of variation [CV (%)] was between 4.5 and 7.91%. Parallel analysis of muscle samples from chickens fed ENR showed good comparable results obtained from the test strip and LC-MS. Each test requires 5-10 min. The data indicate that the method has high sensitivity, specificity, and the advantages of simplicity and speed of performance. Therefore, the test strip provides a useful screening method for quantitative, semiquantitative, or qualitative detection of ENR residues in chicken muscles.

  • 相关文献

[1]Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Zhang, G.,Wang, X.,Bao, Y.,Wang, Z.,Zhang, G.,Wang, X.,Yang, Y.,Luo, J.,Li, Q.,Guo, J.,Yang, J.,Xing, G.,Chai, S.,Shi, T.,Liu, Q.,Zhi, A.,Qu, M.. 2008

[2]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues. Song, Chunmei,Liu, Qingtang,Zhi, Aimin,Yang, Jifei,Zhi, Yubao,Li, Qingmei,Hu, Xiaofei,Deng, Ruiguang,Zhang, Gaiping,Song, Chunmei,Casas, Justin,Tang, Liang.

[3]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[4]Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn. Sun, Yaning,Hu, Xiaofei,Yang, Jifei,Wang, Fangyu,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[5]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[6]Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test. Wang, Yao,Li, Zhixi,Deng, Ruiguang,Zhang, Gaiping,Li, Qingmei,Yang, Jifei,Sun, Yaning,Hu, Xiaofei,Zhang, Gaiping.

[7]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[8]Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen beta-Conglycinin. Wang, Yao,Li, Zhaozhou,Pei, Yafeng,Hou, Yuze,Wang, Yao,Li, Qingmei,Sun, Yaning,Yang, Jifei,Yang, Yanyan,Zhi, Yubao,Deng, Ruiguang,Hu, Xiaofei.

[9]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

[10]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[11]Development of a one-step strip test for the diagnosis of chicken infectious bursal disease. Zhang, GP,Li, QM,Yan, YY,Guo, JQ,Li, XW,Deng, RG,Xiao, ZJ,Xing, GX,Yang, JF,Zhao, D,Cai, SJ,Zang, WM. 2005

[12]Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize. Yao, Jingjing,Sun, Yaning,Li, Qingmei,Wang, Fangyu,Teng, Man,Yang, Yanyan,Deng, Ruiguang,Hu, Xiaofei.

[13]Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Hua, Xiude,Qian, Guoliang,Hu, Baishi,Fan, Jiaqin,Qin, Na,Li, Gang,Wang, Yuyan,Liu, Fengquan,Yang, Jifei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台