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Effects of 1-MCP and ethylene on expression of three CAD genes and lignification in stems of harvested Tsai Tai (Brassica chinensis)

文献类型: 外文期刊

作者: Zhang, Lubin 1 ; Wang, Guang 1 ; Chang, Jinmei; Liu, Jianshuang 1 ; Cai, Jianghua 1 ; Rao, Xiuwen 1 ; Zhang, Lijuan 1 ;

作者机构: 1.S China Agr Univ, Guangdong Prov Key Lab Postharvest Physiol & Tech, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China

2.Chinese Acad Tro

关键词: cinnamyl alcohol dehydrogenase;ethylene;1-MCP;senescence;lignification;brassica chinensis

期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Tsai Tai (Brassica chinensis) is produced mainly in China but also consumed overseas. The stems of Tsai Tai are subject to toughening due to lignification. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme involved in lignin biosynthesis. Three full-length CAD cDNAs were isolated, encoding proteins of 288, 323, and 323 amino acids, respectively. Sequence analysis showed that they share a highly conserved putative NAD/NADP(H)-binding site at the amino terminus. All the three BcCAD genes responded to 1-MCP and ethylene within 2 h. Ethylene up-regulated expression of BcCADl-1 and BcCAD2, while 1-MCP down-regulated them. Ethylene increased yellow leaves and stem lignin, and 1-MCP decreased them. These results suggest ethylene is involved in lignin growth in Tsai Tai, and induced expression of BcCAD1-l and BcCAD2 could contribute to lignification. They also suggest removal of ethylene and application of 1 -MCP could extend shelf-life and knockout of certain CAD genes could produce lines that store well.

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