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Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues

文献类型： 外文期刊

作者： Song, Chunmei 1 ; Liu, Qingtang 1 ; Zhi, Aimin 1 ; Yang, Jifei 1 ; Zhi, Yubao 1 ; Li, Qingmei 1 ; Hu, Xiaofei 1 ; Deng, R 1 ;

作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

2.Henan Univ Sci & Technol, Food & Bioengn Coll, Luoyang 471003, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX 78249 USA

关键词： olaquindox;test strip;colloidal gold;immunoassay;rapid test

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A rapid immunochromatographic lateral flow test strip of competitive format has been developed for the specific determination of olaquindox (OLA) residues in pig urine and muscle tissues. The sensitivity of the test strip was found to be 1.58 ± 0.27 μg/kg and 1.70 ± 0.26 μg/kg of OLA in pig urine and muscle tissues, and the lower detection limit was 0.27 ± 0.08 μg/kg and 0.31 ± 0.07 μg/kg respectively. For negative pig urine and muscle samples spiked with 4, 12, and 36 μg/kg, the recovery range was 83.0-94.0% and 78.8-87.4% and the coefficient of variation scope [CV (%)] was 3.17-7.41% and 4.66-7.6496 respectively. Parallel analysis of OLA samples from pig urine and muscle tissue showed comparable results from the test strip and HPLC. Each test requires 5-8 min, and the test strip can provide a useful screening method for quantitative, semiquantitative, or qualitative detection of OLA residues.

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