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Functional annotation of an expressed sequence tag library from Haliotis diversicolor and analysis of its plant-like sequences

文献类型： 外文期刊

作者： Jiang, Jing-Zhe 1 ; Zhang, Wei 2 ; Guo, Zhi-Xun 1 ; Cai, Chen-Chen 3 ; Su, You-Lu 1 ; Wang, Rui-Xuan 1 ; Wang, Jiang-Y 1 ;

作者机构： 1.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Dept Equipment & Lab Management, Guangzhou 510275, Guangdong, Peoples R China

3.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China

关键词： Small abalone;Haliotis diversicolor;Expressed sequence tag;Complementary DNA;Library;Plant-like gene

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

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收录情况： SCI

摘要： The small abalone, Haliotis diversicolor, is a widely distributed and cultured species in the subtropical coastal area of China. To identify and classify functional genes of this important species, a normalized expressed sequence tag (EST) library, including 7069 high quality ESTs from the total body of H. diversicolor, was analyzed. A total of 4781 unigenes were assembled and 2991 novel abalone genes were identified. The GC content, codon and amino acid usage of the transcriptome were analyzed. For the accurate annotation of the abalone library, different influencing factors were evaluated. The gene ontology (GO) database provided a higher annotation rate (69.6%), and sequences longer than 800 bp were easily subjected to a BLAST search. The taxonomy of the BLAST results showed that lancelet and invertebrates are most closely related to abalone. Sixty-seven identified plant-like genes were further examined by reverse transcription–polymerase chain reaction (RT–PCR) and sequencing, only seven of these were real transcripts in abalone. Phylogenic trees were also constructed to illustrate the positions of two Cystatin sequences and one Calmodulin protein sequence identified in abalone. To perform functional classification, three different databases (GO, KEGG and COG) were used and 60 immune or disease-related unigenes were determined. This work has greatly enlarged the known gene pool of H. diversicolor and will have important implications for future molecular and genetic analyses in this organism.

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