您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

cDNA cloning and characterization of a new member of the tumor necrosis factor receptor family gene from scallop, Chlamys farreri

文献类型： 外文期刊

作者： Su, Jianguo 1 ; Qiu, Limei 1 ; Li, Ling 1 ; Liu, Lin 1 ; Wang, Lingling 1 ; Siva, Vinu S. 1 ; Yu, Dahui 3 ; Song, Linshen 1 ;

作者机构： 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China

2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

关键词： cDNA: complementary DNA;osteoprotegerin: 205944-50-9;mRNA;tumor necrosis factor receptor: TNFR;apoptosis;autoimmunity;organogenesis;immune defense;species evolutionary origin

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Tumor necrosis factors receptor (TNFR) is a superfamily of proteins derived mainly from vertebrates. It plays significant role in diverse physiological and pathological events such as inflammation, apoptosis, autoimmunity and organogenesis. The gene of a new member of TNFR family, designated as CfTNFR2, was cloned and characterized from scallop, Chlamys farreri. The full-length cDNA of CfTNFR2 consisted of 1,501 nucleotides with a poly (A) tail, encoding a polypeptide of 378 amino acids with the estimated molecular mass of 42.70 kDa and predicted isoelectric point of 4.79. The characteristic motifs of the TNFR family proteins, such as three TNFR homology domains (also called CRD domains) and a death domain, were identified in CfTNFR2. Significantly, the deduced amino acid sequence of CfTNFR2 was closely homologous with mammalian osteoprotegerins showing approximately 37% identity. However, it shared only 11% amino acids identity with CfTNFR1, another TNFR homolog previously identified from the candidate scallop species, indicating that CfTNFR2 is a new molluscan TNFR protein. The spatial expression of CfTNFR2 in the tissues of the healthy and bacterial challenged scallops was detected by real-time PCR. CfTNFR2 mRNA was expressed constitutively in all selected tissues such as mantle, gill, gonad, hepatopancreas and hemocyte, among which gill and mantle displayed comparatively higher expression levels. Upon Listonella anguillarum challenge, CfTNFR2 expression was found to be remarkably up-regulated, especially in the tissues of gill (15.9-fold) and mantle (8.0-fold). The results reveal that CfTNFR2 is a constitutive and inducible acute-phase protein apparently involved in immune defense. The presence of CfTNFR2 (present study) and CfTNFR1 (previously identified from our lab) encouraged us to suggest that multiple members of TNFR family exist in mollusk, and the findings would help us to get better understanding on the evolutionary origin and functions of this protein family in mollusks.

  • 相关文献

[1]Identification and involvement of ferritin in the response to pathogen challenge in the abalone, Haliotis diversicolor. He, Jian,Jiang, Jingzhe,Gu, Lu,Zhao, Manman,Wang, Ruixuan,Ye, Lingtong,Yao, Tuo,Wang, Jiangyong,Gu, Lu,Zhao, Manman.

[2]Morphological development and growth of the freshwater mussel Anodonta woodiana from early juvenile to adult. Chen, Xiubao,Liu, Hongbo,Su, Yanping,Yang, Jian.

[3]Cloning and analysis of the full-length Rubisco large subunit (rbcL) cDNA from Ulva linza (Chlorophyceae, Chlorophyta). Ying, Cheng-Qi,Yin, Shun-Ji,Shen, Yi,He, Pei-Min,Ying, Cheng-Qi,Lin, Sen-Jie.

[4]Cloning and characterization of a beta-1,3-glucan-binding protein from shrimp Fenneropenaeus chinensis. Lai, Xiaofang,Kong, Jie,Wang, Qingyin,Wang, Weiji,Meng, Xianhong,Lai, Xiaofang,Lai, Xiaofang.

[5]A new Akirin1 gene in turbot (Scophthalmus maximus): Molecular cloning, characterization and expression analysis in response to bacterial and viral immunological challenge. Yang, Chang-Geng,Wang, Xian-Li,Wang, Lei,Zhang, Bo,Chen, Song-Lin,Yang, Chang-Geng.

[6]Identification and expression analysis of two growth hormone receptors in zanzibar tilapia (Oreochromis hornorum). Gao, Feng-Ying,Lu, Mai-Xin,Ye, Xing,Huang, Zhang-Han,Wang, Huan,Zhu, Hua-Ping,Yang, Li-Ping,Wang, Huan.

[7]尼罗罗非鱼外周血白细胞总RNA的分离及反转录验证. 李超,陈明,黄维义,王瑞,余晓丽,黄婷,雷爱莹,梁万文. 2010

[8]基于病毒mRNA监测的传染性脾肾坏死病毒灭活快速检验方法建立及其应用. 张醴溪,杨圆圆,方伟,付小哲,林强,李趁,刘礼辉,梁红茹,黄志斌,吴志新,李宁求. 2017

[9]壳聚糖硫酸酯提高异育银鲫抗嗜水气单胞菌感染的研究. 周帅,周常,房文红. 2010

[10]Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Gu, Zhimin,Xuan, Fujun.

[11]Effects of temperature and dietary protein on the growth performance and IGF-I mRNA expression of juvenile mirror carp (Cyprinus carpio). Huang, J. F.,Xu, Q. Y.,Chang, Y. M.,Huang, J. F..

[12]Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Wang, CY,Duan, YR,Markovic, B,Barbara, J,Howlett, CR,Zhang, XD,Zreiqat, H. 2004

[13]Effect of high dietary carbohydrate on growth, serum physiological response, and hepatic heat shock cognate protein 70 expression of the top-mouth culter Erythroculter ilishaeformis Bleeker. Liu, Bo,Xie, Jun,Ge, Xian-ping,Wang, Guangyu,Liu, Bo,Xie, Jun,Ge, Xian-ping,Miao, Ling-hong.

[14]Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Ma, Jianjun,Jiang, Jingjing,Pan, Dequan,Zhang, Dianchang,Jiang, Jingjing.

[15]Molecular and expression characterization of growth hormone/prolactin family genes in the Prenant's schizothoracin. Li, Chuangju,Chen, Xihua,Zhang, Yan,Ye, Huan,Liu, Tao,Li, Chuangju,Zhang, Yan.

[16]Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Tan, Aiping,Bai, Junjie,Li, Shengjie,Zhang, Lili.

[17]The effect of emodin on cytotoxicity, apoptosis and antioxidant capacity in the hepatic cells of grass carp (Ctenopharyngodon idellus). Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan,Cui, Yan-ting,Liu, Bo,Xie, Jun,Xu, Pao,Habte-Tsion, H. -Michael,Zhang, Yuan-yuan.

[18]Effects of carbon tetrachloride on oxidative stress, inflammatory response and hepatocyte apoptosis in common carp (Cyprinus carpio). Jia, Rui,Wang, Jia-Hao,Liu, Ying-Juan,Xu, Pao,Yin, Guo-Jun,Jia, Rui,Cao, Li-Ping,Du, Jin-Liang,Xu, Pao,Yin, Guo-Jun,Cao, Li-Ping,Du, Jin-Liang,Yin, Guo-Jun,Jeney, Galina.

[19]Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-8 from the blunt snout bream (Megalobrama amblycephala) exposed to ammonia. Sun, Shengming,Ge, Xianping,Zhu, Jian,Zhang, Wuxiao,Zhang, Qiong.

[20]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台