Biochemical characterization of a proteoglycan complex from an edible mushroom Ganoderma lucidum fruiting bodies and its immunoregulatory activity
文献类型: 外文期刊
作者: Ye, LiBin 1 ; Zheng, Xiaolin 1 ; Zhang, Jingsong 2 ; Tang, Qingqiu 2 ; Yang, Yan 2 ; Wang, Xiangyang 1 ; Li, Jianrong; 1 ;
作者机构: 1.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
3.Shanghai Ocean Univ, Coll Food Sci, Shanghai 200090, Peoples R China
关键词: ganoderma lucidum;proteoglycan;immunomodulation;b cells
期刊名称:FOOD RESEARCH INTERNATIONAL ( 影响因子:6.475; 五年影响因子:6.508 )
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收录情况: SCI
摘要: A fraction, LZ-D-7, was isolated from the fruiting body of the edible mushroom Ganoderma lucidum using a series of chromatographic steps. Biochemical experiments showed that the complex is a proteoglycan and has a carbohydrate percentage of 953%, along with a protein percentage of 3.3%. Methylation analysis showed the polysaccharide moiety of LZ-D-7 had a backbone of 1,4-disubsituted-glucopyranosyl and 1,2,6-trisubstituted-glucopyranosyl residues. The branches were mainly composed of 1-substituted-glucopyranosyl residue, which was attached to 1,2,6-trisubstituted-glucopyranosyl residue via 2 or 6 position. Investigation of modulation of LZ-D-7 on BALB/c mouse spleen lymphocytes cells resulted in a three to four-fold increase of MSLs percentage. Analysis of activation and proliferation of MSLs indicated that LZ-D-7 activating most of the activated cells were B cells. The B cells were enlarged, expressed CD~(71+) and CD~(25+) on the cell surface.
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