Isolation of Clostridium perfringens strain W11 and optimization of its biohydrogen production by genetic modification
文献类型： 外文期刊
作者： Wang, Ruofan 1 ; Zong, Wenming 2 ; Qian, Changli 1 ; Wei, Yongjun 1 ; Yu, Ruisong 1 ; Zhou, Zhihua 1 ;
作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China
2.Anhui Agr Univ, Coll Engn, Hefei 230036, Peoples R China
3.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Med, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： clostridium perfringens;biohydrogen;genetic modification;lactate dehydrogenase
期刊名称：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY （ 影响因子：5.816； 五年影响因子：5.242 ）
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收录情况： SCI
摘要： Clostridium perfringens strain Wll, which we previously identified as the major hydrogen producer in a hydrogen-producing microbial flora, was isolated in this study. The hydrogen yield from sucrose of this strain was 1.S3 mol H_2/mol hexose. To exclude potential safety problems, the pic gene, encoding an alpha toxin protein, was permanently knocked out using the Targetron gene knockout system, creating strain W12. Strains Wll and W12 both produced lactate, acetate, and butyrate during hydrogen production. Furthermore, yields of these metabolites and hydrogen were near-identical by the two strains. When the Idh gene encoding lactate dehydrogenase in strain W12 was deleted, the hydrogen yield and acetate and butyrate concentrations in the resulting mutant, W13, increased by 51%, 26%, and 57%, respectively. Lactate production by strain W13 decreased almost to zero. The growth rates of the wild-type strain Wll and its mutant derivatives were similar.
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