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Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections

文献类型： 外文期刊

作者： Yang, Zhenhua 1 ; Wu, Rui 1 ; Li, Robert W. 2 ; Li, Ling 1 ; Xiong, Zhongliang 3 ; Zhao, Haizhong 3 ; Guo, Deyin 1 ; Pan, 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

2.ARS, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, Peoples R China

关键词： Chimeric;Classical swine fever virus;Japanese encephalitis virus;Trans-complementation

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： A trans-complemented chimeric CSF-JE virus replicon was constructed using an infectious cDNA clone of the CSF virus (CSFV) Alfort/187 strain. The CSFVE2 gene was deleted, and a fragment containing the region encoding a truncated envelope protein (tE, amino acid 292-402, domain III) of JE virus (JEV) was inserted into the resultant plasmid, pA187delE2, to generate the recombinant cDNA clone pA187delE2/JEV-tE. Porcine kidney 15 (PK15) cells that constitutively express the CSFV E2p7 proteins were then transfected with in vitro-transcribed RNA from pA187delE2/JEV-tE. As a result, the chimeric CSF-JE virus replicon particle (VRP), rv187delE2/JEV-tE, was rescued. In a mouse model, immunization with the chimeric CSF-JE VRP induced strong production of JEV-specific antibody and conferred protection against a lethal JEV challenge. Pigs immunized with CSF-JE VRP displayed strong anti-CSFV and anti-JEV antibody responses and protection against CSFV and JEV challenge infections. Our evidence suggests that E2-complemented CSF-JE VRP not only has potential as a live-attenuated non-transmissible vaccine candidate against CSF and JE but also serves as a potential DIVA (Differentiating Infected from Vaccinated Animals) vaccine for CSF in pigs. Together, our data suggest that the non-transmissible chimeric VRP expressing foreign antigenic proteins may represent a promising strategy for bivalent DIVA vaccine design.

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[1]Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice. Wu, Rui,Tian, Yongxiang,Deng, Junhua,Yang, Keli,Liang, Wangwang,Guo, Rui,Duan, Zhengying,Liu, Zewen,Zhou, Danna,Xu, Diping,Wu, Rui,Tian, Yongxiang,Xu, Diping,Wu, Rui. 2011

[2]Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Liao, Yonghong,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Zhang, Tao,Wen, Guoyuan,Hu, Zhibin,Song, Nianhua,Zhang, Tao. 2011

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