The recombinant E-rns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus
文献类型： 外文期刊
作者： Yi, Weicheng 1 ; Zhu, Hongchang 1 ; Wu, Yihan 1 ; Li, Qingmei 2 ; Lou, Wange 1 ; Zhao, Haizhong 3 ; Pan, Zishu 1 ;
作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
3.Hubei Acad Agr Sci, Inst Anim Husb & Vet Med, Wuhan 430064, Peoples R China
关键词： Classical swine fever virus; Bovine viral diarrhea virus; E-rns; E2; Serological diagnosis; Indirect enzyme-linked immunosorbent assay
期刊名称：VIROLOGY JOURNAL （ 影响因子：5.913； 五年影响因子：4.372 ）
ISSN：
年卷期： 2022 年 19 卷 1 期
页码：
收录情况： SCI
摘要： Background Classical swine fever (CSF) virus is the causative agent of an economically important, highly contagious disease of pigs. CSFV is genetically and serologically related to bovine viral diarrhea virus (BVDV). BVDV infection in pigs can mimic CSF clinical signs, which cause difficulty in differentiation. Serological test for detection of virus specific antibodies is a valuable tool for diagnosis and surveillance of CSFV and BVDV infections in animals. The aim of this study was to develop the CSFV E-rns and BVDV tE2 -based ELISAs to distinguishably test specific antibodies against CSFV and BVDV. Methods The CSFV E-rns and truncated E2 (tE2, residues 690-865) of BVDV were expressed in E. coli and purified by Ni-NTA affinity chromatography, respectively. Employing E-rns or tE2 protein as diagnostic antigen, indirect ELISAs were developed to distinguishably test specific antibodies against CSFV and BVDV. The specificity and sensitivity of ELISAs were evaluated using a panel of virus specific sera of pigs, immunized rabbits and immunized mice. A total 150 clinical serum samples from farm pigs were measured by the developed ELISAs and compared with virus neutralizing test (VNT). Results Indirect ELISA was established based on recombinant CSFV E-rns or BVDV tE2 protein, respectively. No serological cross-reaction between antibodies against CSFV and BVDV was observed in sera of immunized rabbits, immunized mice or farm pigs by detections of the E-rns and tE2 -based ELISAs. Compared to VNT, the CSFV E-rns -based ELISA displayed a high sensitivity (93.3%), specificity (92.0%) and agreement rate (92.7%), and the sensitivity, specificity and agreement rate of BVDV tE2 -based ELISA was 92.3%, 95.2% and 94.7%, respectively. Conclusion The newly developed ELISAs are highly specific and sensitive and would be valuable tools for serological diagnosis for CSFV and BVDV infections.
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