Characterization of thermostable Newcastle disease virus recombinants expressing the hemagglutinin of H5N1 avian influenza virus as bivalent vaccine candidates
文献类型： 外文期刊
作者： Xu, Lulai 1 ; Qin, Zhenqiao 1 ; Qiao, Lei 1 ; Wen, Jie 1 ; Shao, Huabin 2 ; Wen, Guoyuan 2 ; Pan, Zishu 1 ;
作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China
2.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr, Wuhan 430064, Peoples R China
关键词： Newcastle disease virus; Hemagglutinin; Highly pathogenic avian influenza H5N1 virus; Vector vaccine; Thermostability
期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）
ISSN： 0264-410X
年卷期： 2020 年 38 卷 7 期
页码：
收录情况： SCI
摘要： Newcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian influenza virus (HPAIV) H5N1 based on the full-length cDNA clone of thermostable TS09-C strain. The recombinant thermostable Newcastle disease viruses, rTS-HA, rTS-HA1 and rTS-tPAs/HA1, expressed the HA, HA1 or modified HA1 protein with the tissue plasminogen activator signal sequence (tPAs), respectively. The rNDVs displayed similar thermostability, growth kinetics and pathogenicity compared with the parental TS09-C virus. The tPAs facilitated the expression and secretion of HA1 protein in cells infected with rNDV. Animal studies demonstrated that immunization with rNDVs elicited effective H5N1- and NDV-specific antibody responses and conferred immune protection against lethal H5N1 and NDV challenges in chickens and mice. Importantly, vaccination of rTS-tPAs/HA1 resulted in enhanced protective immunity in chickens and mice. Our study thus provides a novel thermostable NDV-vectored vaccine candidate expressing a soluble form of a heterologous viral protein, which will greatly aid the poultry industry in developing countries. (C) 2019 Elsevier Ltd. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Research note: Prevalence and genetic characteristics of pathogenic E. coli isolates from domestic pigeons in central China
作者：Wang, Juan;Lu, Qin;Yao, Lun;Zhang, Wenting;Hu, Qiao;Guo, Yunqing;Wen, Guoyuan;Shao, Huabin;Luo, Qingping;Zhang, Tengfei;Wen, Guoyuan;Luo, Qingping
关键词：Pathogenic E. coli; Pigeons; Serotypes; Antibiotic resistance
-
Construction and bacteriostatic effect analyses of a recombinant thermostable Newcastle disease virus expressing cecropin AD
作者：Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Ren, Xiangfei;Yang, Junjie;Hu, Sishun;Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zhou, Ping;Zeng, Chi;Luo, Qingping
关键词：Newcastle disease virus; Cecropin AD; Staphylococcus aureus; Wound healing
-
Addressing unexpected bacterial RNA safety concerns of E. coli produced influenza NP through CpG loaded mutant
作者：Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhang, Wanpo;Gu, Changqin;Zhou, Hongbo;Tao, Pan;Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhou, Hongbo;Tao, Pan;Wen, Guoyuan
关键词：
-
Genetic and pathogenic characteristics of a novel recombinant GI-19 infectious bronchitis virus strain isolated from northeastern China
作者：Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Feng, Helong;Zhou, Chengli
关键词：Infectious bronchitis virus; GI-19; Evolutionary; Recombination; Pathogenicity; Antigenic variation
-
The impacts of animal agriculture on One Health-Bacterial zoonosis, antimicrobial resistance, and beyond
作者：Zhang, Tengfei;Zhang, Wenting;Shang, Yu;Luo, Qingping;Wen, Guoyuan;Nickerson, Rhea;Cheng, Zhenyu;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Luo, Qingping
关键词：Zoonosis; Antimicrobial resistance
-
BacScan: a novel genome-wide strategy for uncovering broadly immunogenic proteins in bacteria
作者：Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Qin, Huan;Wen, Guoyuan
关键词：bacterial subunit vaccine; vaccine target; immunogenic protein; protective antigen; genome-wide screening; streptococcus suis
-
Spray vaccination with a Newcastle disease virus (NDV)-vectored infectious laryngotracheitis (ILT) vaccine protects commercial chickens from ILT in the presence of maternally-derived antibodies
作者：Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan
关键词：ILTV; NDV; vector vaccine; spray vaccination; maternally derived antibodies; protection
