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Enzyme microcapsules with substrate selective permeability constructed via layer-by-layer polyelectrolyte self-assembly

文献类型： 外文期刊

作者： Zhao, Zi-Xia 2 ; Wang, Xin-Sheng 1 ; Qin, Xia 1 ; Chen, Qiang 1 ; Anzai, Jun-ichi 3 ;

作者机构： 1.Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Bioact Mat, Tianjin 300071, Peoples R China

2.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

3.Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi 9808578, Japan

关键词： Microcapsules;Enzyme;Polyelectrolyte;Layer-by-layer self-assembly;Permeability

期刊名称：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS （ 影响因子：7.328； 五年影响因子：6.654 ）

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收录情况： SCI

摘要： Polyelectrolyte multilayer microcapsules with entrapped horseradish peroxidase (HRP) have been prepared via a layer-by-layer (LbL) self-assembly strategy of polycation and polyanion on CaCO_3 microparticles as templates. Preparation conditions have been studied and optimized. Within the investigated ranges, use of buffer solutions with lower pH value or lower ionic strength in the buffer solution for polyelectrolyte self-assembly has resulted in thinner polyelectrolyte film and higher permeability of the microcapsules. For dissolving the CaCO_3 templates, use of weakly acidic (pH 4.0) buffer solution in place of routinely used EDTA reagent has improved the catalytic activity of microcapsules. The HRP-containing microcapsules have exhibited catalytic activity to pyrogallol as substrate, while the catalytic activity to 2,2′-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) was severely suppressed. No less than 90% of the maximum enzymatic activity to pyrogallol has remained after 30 days. Promising prospects in various biocatalysis applications have been expected for the enzyme microcapsules, due to their selective permeability, stability and reusability.

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