文献类型： 外文期刊
作者： Xiong, Ai-Sheng 1 ; Peng, Ri-He 2 ; Zhuang, Jing 3 ; Davies, Jim 3 ; Zhang, Jian 3 ; Yao, Quan-Hong 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China
3.Alberta Res Council, Alberta Innovates Technol Futures, Vegreville, AB T9C 1T4, Canada
期刊名称：CRITICAL REVIEWS IN BIOTECHNOLOGY （ 影响因子：8.429； 五年影响因子：8.981 ）
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收录情况： SCI
摘要： Many reporter genes, such as gfp, gusA, and lacZ, are widely used for research into plants, animals, and microorganisms. Reporter genes, which offer high levels of sensitivity and convenience of detection, have been utilized in transgenic technology, promoter analysis, drug screening, and other areas. Directed molecular evolution is a powerful molecular tool for the creation of designer proteins for industrial and research applications, including studies of protein structure and function. Directed molecular evolution is based mainly on in vitro recombination methods, such as error-prone PCR and DNA shuffling. The strategies of directed evolution of enzyme biocatalysts have been the subject of several recent reviews. Here, we briefly summarize successes in the field of directed molecular evolution of reporter genes and discuss some of the applications.
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