Construction of a human scFv antibody library with VH regions randomized and its application
文献类型： 外文期刊
作者： Liu, Yunchao 2 ; Chang, Junbiao 2 ; Chen, Yumei 1 ; Wan, Bo 1 ; Wang, Yinbiao 1 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
3.Zhengzhou Univ, Zhengzhou 450001, Peoples R China
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
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收录情况： SCI
摘要： A non-immunized human single chain variable fragment (scFv) library containing 2.5 x 10(7) individual clones was constructed from antibody variable region genes of 200 non-immunized donors. ScFv gene repertories were generated by randomly combining rearranged variable regions of heavy chain (VH) and natural occurring light chain (VL) using overlapping extension PCR (OE-PCR). Five recombinant protein antigens from different species were successfully used to select specific binders. Phage ELISA showed that the recombinant phage particle could specifically bind to non-structural protein 1 of Avian influenza virus. This method can therefore efficiently generate a phage antibody library.
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