Construction of a human scFv antibody library with VH regions randomized and its application
文献类型： 外文期刊
作者： Liu, Yunchao 2 ; Chang, Junbiao 2 ; Chen, Yumei 1 ; Wan, Bo 1 ; Wang, Yinbiao 1 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
3.Zhengzhou Univ, Zhengzhou 450001, Peoples R China
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A non-immunized human single chain variable fragment (scFv) library containing 2.5 x 10(7) individual clones was constructed from antibody variable region genes of 200 non-immunized donors. ScFv gene repertories were generated by randomly combining rearranged variable regions of heavy chain (VH) and natural occurring light chain (VL) using overlapping extension PCR (OE-PCR). Five recombinant protein antigens from different species were successfully used to select specific binders. Phage ELISA showed that the recombinant phage particle could specifically bind to non-structural protein 1 of Avian influenza virus. This method can therefore efficiently generate a phage antibody library.
- 相关文献
作者其他论文 更多>>
-
Potential Pathogenicity and Genetic Characteristics of a Live-Attenuated Classical Swine Fever Virus Vaccine Derivative Variant
作者：Guo, Zhenhua;Xing, Guangxu;Jin, Qianyue;Lu, Qingxia;Zhang, Gaiping;Wang, Leyi;Wang, Leyi;Zhang, Gaiping;Zhang, Gaiping
关键词：
-
Proteomic analysis reveals the antiviral effects of baicalin on pseudorabies virus
作者：Niu, Qiaoge;Zhang, Gaiping;Niu, Qiaoge;Zhou, Chuanjie;Li, Rui;Guo, Junqing;Qiao, Songlin;Chen, Xin-xin;Zhang, Gaiping;Zhang, Gaiping
关键词：Baicalin; Pseudorabies virus; ROS; F3; NFU1; CEBPB
-
Marine peptides as potential anti-aging agents: Preparation, characterization, mechanisms of action, and future perspectives
作者：Yao, Wanzi;Zhang, Yifeng;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：Marine peptides; Anti-aging; Preparation; Mechanisms; Oxidative stress; Signaling pathways
-
Thiolated chitosan encapsulation constituted mucoadhesive nanovaccine confers broad protection against divergent influenza A viruses
作者：Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Chai, Shujun;Zhang, Gaiping;Zhang, Gaiping
关键词：Mucoadhesive; Thiolated chitosan; Influenza nanovaccine; M2e; Nucleoprotein
-
Identification of the Linear Fc-Binding Site on the Bovine IgG1 Fc Receptor (boFcγRIII) Using Synthetic Peptides
作者：Wang, Ruining;Guo, Junqing;Yang, Jifei;Li, Qingmei;Zhang, Gaiping;Wang, Ruining;Li, Ge;Wang, Xun;Zhang, Gaiping;Zhang, Gaiping
关键词：BoFc gamma RIII; Fc-binding site; bovine IgG1; synthetic peptides
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
